Synthesis of a Temperature Responsive Semi-Inter-Penetrating Polymer Network of Poly (Diethyl Acrylamide)-Xanthan Gum Enriched With Umbilical Cord Blood Serum for Wound Healing Publisher



Ranjbar R ; Varshosaz J ; Baneshi F ; Minaiyan M ; Mirian M ; Tabbakhian M ; Esfahani SN
Source: International Journal of Pharmaceutics: X Published:2026

Abstract

Human umbilical cord blood serum (UCBS) represents a potential reservoir of growth factors, neurotrophic factors, and cytokines, with anti-inflammatory properties. This study aimed to synthesize a temperature sensitive interpenetrating polymer network (semi-IPN) of diethyl acrylamide and xanthan gum enriched with UCBS for damaged skin tissue. The advantages of 3D IPN hydrogel are enhanced material-exchange, biocompatibility, and mechanical properties adjustability. The semi-IPN containing 1% xanthan gum exhibited the lower critical solution temperature of about 32 °C. 70% of the loaded UCBS in hydrogel was released within 24 h. The semi-IPN dramatically promoted cells proliferation and adhesion. 99% wound closure happened by the hydrogel loaded UCBS. Histological and immunohistochemical analyses indicated that the gel reduced inflammation, downregulated surface CD31 markers, increased collagen density, and accelerated re-epithelialization in the healed tissue, suggesting improved tissue regeneration. These findings highlight the strong potential of semi-IPN diethyl acrylamide-xanthan gum hydrogel dressing for effective management of open wounds. © 2026 The Authors.