Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effects of Morphine on Vascular Cell Adhesion Molecule 1(Vcam-1) Concentration in Lung Cancer Cells Publisher Pubmed



Ghasemi A1 ; Vaseghi G2 ; Hojjatallah A3 ; Haghjooy Javanmard S1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Physiology, School of medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Archives of Physiology and Biochemistry Published:2023


Abstract

Objective: Vascular cell adhesion molecule 1 (VCAM-1) plays an important role in tumour cell adhesion to endothelial cells. Some tumour cells also show aberrant expression of VCAM‐1. Toll-like receptor 4 (TLR4) agonists can increase VCAM-1 expression. Morphine, an opioid receptor agonist, is also a TLR4 agonist. In this study, we aimed to evaluate whether morphine increase VCAM-1 expression in a TLR4 dependent manner. Methods: A549 Lung cancer cells were treated with different doses of morphine and TLR4 antagonist for 24 and 48 h. TLR4 gene expression was evaluated by real-time PCR and VCAM-1 protein was measured by the enzyme-linked immunosorbent assay (ELISA). Results: Morphine enhanced mRNA expression of TLR4 and protein level of VCAM-1. TLR4 antagonist returned VCAM-1expression to the normal level. Conclusion: Morphine effects VCAM-1expressions via TLR4 in lung cancer cell line. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
View other Related Docs
1. Morphine Promotes Migration and Lung Metastasis of Mouse Melanoma Cells, Brazilian Journal of Anesthesiology (English Edition) (2023)
2. Morphine Upregulates Toll-Like Receptor 4 Expression and Promotes Melanomas in Mice, Immunopharmacology and Immunotoxicology (2023)
3. The Potential Interplay Between Opioid and the Toll-Like Receptor 4 (Tlr-4), Immunopharmacology and Immunotoxicology (2023)
4. Influence of Morphine on Tlr4/ Nf-Kb Signaling Pathway of Mcf-7 Cells, Bratislava Medical Journal (2018)
5. Effects of Methadone on the Toll-Like Receptor 4 Expression in Human Non-Small Cell Lung Carcinoma A549 Cell Line Using In-Silico and in Vitro Techniques, Advanced Biomedical Research (2022)
6. Effects of the Co-Administration of Morphine and Lipopolysaccharide on Toll-Like Receptor-4/Nuclear Factor Kappa Β Signaling Pathway of Mda-Mb-231 Breast Cancer Cells, Advanced Biomedical Research (2023)
7. Influences of Morphine on Cannabinoid Receptor 1, 2 Expression in Breast Cancer Cell Lines, International Journal of Women's Health and Reproduction Sciences (2024)
8. Effect of Lipopolysaccharide on Toll-Like Receptor-4 Signals in Mouse Cancer Cells, Bratislava Medical Journal (2017)
Experts (# of related papers)
View all Related Experts
Golnaz Vaseghi (11)
Shaghayegh Haghjooy Javanmard (10)
Other Related Docs
9. Ppar Γ Agonist, Pioglitazone, Suppresses Melanoma Cancer in Mice by Inhibiting Tlr4 Signaling, Journal of Pharmacy and Pharmaceutical Sciences (2019)
10. The Effect of Fenofibrate, a Pparα Activator on Toll-Like Receptor-4 Signal Transduction in Melanoma Both in Vitro and in Vivo, Clinical and Translational Oncology (2020)
11. In Silico Interactions of Morphine Opioids With Tlr4 and Trpv1 Targets, Biointerface Research in Applied Chemistry (2023)
12. Evaluation of Effects of Morphine and Ionizing Radiation in Cancer Cell Lines, Journal of Cancer Research and Therapeutics (2019)