Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
In Vitro Evaluation of the Antibacterial Activity of Five Sealers Used in Root Canal Therapy Publisher



Hasheminia M1 ; Razavian H1 ; Mosleh H2 ; Shakerian B3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Dental Materials Research Center, Department of Endodontics, School of Dentistry, Isfahan University of Medical Sciences, Hezar Jerib St., Isfahan, Iran
  2. 2. Department of Pediatric Dentistry, School of Dentistry, Isfahan University of Medical Sciences, Iran
  3. 3. Dentist, Isfahan, Iran

Source: Dental Research Journal Published:2017


Abstract

Background: Antibacterial activity is one of the important characteristics of an ideal root canal sealer. The aim of this study was to investigate the antibacterial activity of five different sealers against Enterococcus faecalis using two different methods. Materials and Methods: The mineral trioxide aggregate (MTA) Fillapex, Tg-sealer, Endomethasone, AH-26, and RoekoSeal sealers were placed into the brain heart infusion (BHI) culture medium containing E. faecalis (PTCC1393). The diameter of the bacterial zone of inhibition was measured. In the direct contact test, a suspension containing grinded set sealers and E. faecalis bacteria was cultured in BHI after 6, 15, and 60 min. The number of colonies in milliliter was calculated. Data were subjected to one-way ANOVA and Tukey's multiple comparisons test (P < 0.05). Results: In the agar diffusion test, Endomethasone had the highest antibacterial activity against E. faecalis compared to other sealers (P < 0.001). In the direct test, the antibacterial effect of MTA Fillapex was significantly higher than that of all other sealers (P < 0.001). Conclusion: The technique and components of the tested sealers affect the antibacterial activity results. This study showed that all of sealers had antimicrobial effect.
Related Docs
View other Related Docs
1. Antimicrobial Activity of a New Nanobased Endodontic Irrigation Solution: In Vitro Study, Dental Hypotheses (2012)
2. Comparative Evaluation of Enterococcus Faecalis Counts in Different Tapers of Rotary System and Irrigation Fluids: An Ex Vivo Study, Dental Research Journal (2018)
3. Effect of a Separated Rotary Instrument on Bacterial Penetration of Obturated Root Canals, Journal of Clinical Dentistry (2006)
4. Synergistic Antibacterial Activity of Chlorhexidine and Hydrogen Peroxide Against Enterococcus Faecalis, Journal of oral science (2013)
5. Comparison of Reciproc, Wave One, Protaper, and One Shape Rotary Instruments in Reduction of Bacterial Load in Root Canals, Giornale Italiano di Endodonzia (2020)
6. Antibacterial Effect of Nano-Chlorhexidine on Enterococcus Faecalis Biofilm in Root Canal System:An in Vitro Study, Dental Research Journal (2022)
7. Comparison of Mta, Cem Cement, and Biodentine As Coronal Plug During Internal Bleaching: An in Vitro Study, International Journal of Dentistry (2020)
8. Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus Faecalis: An in Vitro Study, Journal of Lasers in Medical Sciences (2020)
Experts (# of related papers)
View all Related Experts
Abbasali Khademi (8)
Masoud Saatchi (6)
Other Related Docs
9. Effect of Nebulized Eucalyptus on Contamination of Microbial Plaque of Endotracheal Tube in Ventilated Patients, Iranian Journal of Nursing and Midwifery Research (2016)
10. Sealing Ability of Mta and Cem Cement As Root-End Fillings of Human Teeth in Dry, Saliva or Bloodcontaminated Conditions, Iranian Endodontic Journal (2010)
11. Antimicrobial Efficacy of 2.5% Sodium Hypochlorite, 2% Chlorhexidine, and 1.5% Hydrogen Peroxide on Enterococcus Faecalis in Pulpectomy of Necrotic Primary Teeth, Journal of Dental Materials and Techniques (2021)
12. Mechanical or Cold Lateral Compaction: The Incidence of Dentinal Defects, Dental Research Journal (2015)
13. The Effect of Various Concentrations of Iodine Potassium Iodide on the Antimicrobial Properties of Mineral Trioxide Aggregate - a Pilot Study, Dental Traumatology (2012)
14. The Effect of Calcium Hydroxide on the Apical Microleakage of Canals Filled With Bioceramic and Resin Sealers, Giornale Italiano di Endodonzia (2020)
15. Inhibitory Effects of Ethanolic, Methanolic, and Hydroalcoholic Extracts of Olive (Olea Europaea) Leaf on Growth, Acid Production, and Adhesion of Streptococcus Mutans, Dental Research Journal (2020)
16. Antibacterial Effect of Calcium Hydroxide Combined With Chlorhexidine on Enterococcus Faecalis: A Systematic Review and Meta-Analysis, Journal of Applied Oral Science (2014)
17. The Effect of Contact With Moisture on the Voids in the Sealer During Canal Obturation With Lateral Condensation Method: An In-Vitro Study, Journal of International Oral Health (2016)
18. Comparative Evaluation of the Cytotoxicity of Two Materials: Calcium Hydroxide Powder, Calcium Hydroxide Reinforced With Chlorhexidine Onl929 Fibroblasts by Mtt Assay, Journal of Kerman University of Medical Sciences (2015)
19. A Comparative Evaluation of the Antimicrobial Effect of Chamomile, Aloe Vera-Green Tea, and Chlorhexidine Mouthwashes on Some Oral Bacterial Species, Dental Research Journal (2023)
20. Apical Microleakage Assessment of Calcium Silicate-Based and Resin-Based Sealers by Fluid Filtration Technique, Journal of Dental Materials and Techniques (2022)
21. Chemical Composition and Antibacterial Activities of Sumac Fruit (Rhus Coriaria) Essential Oil on Dental Caries Pathogens, Open Microbiology Journal (2020)
22. Antimicrobial Efficacy of Antiplaque Agents of Common Toothpastes Against Porphyromonas Gingivalis and Streptococcus Oralis: An in Vitro Study, Dental Research Journal (2024)
23. The Effect of Spraying Different Disinfectants on Condensational Silicone Impressions; an in Vitro Study, Journal of Indian Prosthodontist Society (2015)
24. Evaluation of the Apical Microleakage of Mta Fillapex, Ah26, and Endofill Sealers; [Avaliacao Da Microinfiltracao Apical De Cimentos Mta Fillapex Ah26 E Endofill], Brazilian Dental Science (2020)
25. Propolis: A New Alternative for Root Canal Disinfection, Iranian Endodontic Journal (2012)
26. In Vitro Anti-Bacterial and Anti-Adherence Effects of Lactobacillus Delbrueckii Subsp Bulgaricus on Escherichia Coli, Research in Pharmaceutical Sciences (2013)
27. The Antibacterial Effects of Coffee Extract, Chlorhexidine, and Fluoride Against Streptococcus Mutans and Lactobacillus Plantarum: An in Vitro Study, Dental Research Journal (2019)
28. Prevalence and Antimicrobial Resistance of Bacterial Agents Isolated From the Cases of Dental Caries, International Tinnitus Journal (2023)
29. Antibacterial Effect of Triantibiotic Mixture Versus Calcium Hydroxide in Combination With Active Agents Against Enterococcus Faecalis Biofilm, Dental Materials Journal (2014)
30. Evaluation of Antimicrobial Efficacy of Calcium Hypochlorite As an Endodontic Irrigant on a Mixed-Culture Biofilm: An Ex Vivo Study, Iranian Endodontic Journal (2019)
31. Comparison of the Microleakage of Mineral Trioxide Aggregate, Calcium-Enriched Mixture Cement, and Biodentine Orthograde Apical Plug, Dental Research Journal (2020)
32. The Antimicrobial Effects of Lactobacillus Plantarum and Lactobacillus Rhamnosus Isolated From Local Kefir Buttermilk on Oral Pathogens; [ثر ضد میکروبی الکتوباسیلوس پالنتاروم و الکتوباسیلوس رامنوزوس جداسازی شده از دوغ کفیر بومی اصفهانبر روی پاتوژنهای دهانی], Journal of Isfahan Medical School (2023)
33. Evaluation of Coronal Microleakage of Mineral Trioxide Aggregate Plug-In Teeth With Short Roots Prepared for Post Placement Using Bacterial Penetration Technique, Indian Journal of Dental Research (2016)
34. Comparison of Sealing Ability of Proroot Mineral Trioxide Aggregate, Biodentine, and Ortho Mineral Trioxide Aggregate for Canal Obturation by the Fluid Infiltration Technique, Dental Research Journal (2018)
35. Identification of Infectious Bacteria in Dental Units, International Journal of Environmental Health Engineering (2024)
36. Antibacterial Effects of Hydrogen Peroxide and Silver Composition on Selected Pathogenic Enterobacteriaceae, International Journal of Environmental Health Engineering (2012)
37. Effect of Electrical Heat Carrier Temperature on Bacterial Leakage of Endodontically Treated Teeth Using a Bioceramic Sealer, Clinical and Experimental Dental Research (2025)
38. Comparison of Antibacterial Activity and Fluoride Release in Tooth-Colored Restorative Materials: Resin-Modified Glass Ionomer, Zirconomer, Giomer, and Cention N, Dental Research Journal (2022)
39. Comparing the Sealing Properties of Mineral Trioxide Aggregate and an Experimental Ceramic Based Root End Filling Material in Different Environments, Indian Journal of Dental Research (2013)
40. The Effect of Er:Yag Laser on Entroccocus Faecalis Bacterium in the Pulpectomy of Anterior Primary Teeth, Journal of Lasers in Medical Sciences (2017)
41. Evaluation of Antibacterial Effect of Propolis and Its Application in Mouthwash Production, Frontiers in Dentistry (2019)
42. Evaluation of Preventive Antibacterial Properties of a Glass-Ionomer Cement Containing Purified Powder of Salvia Officinalis: An in Vitro Study, International Journal of Preventive Medicine (2019)
43. Effect of Different Separated Nickel-Titanium Rotary Instruments on Apical Microleakage: An in Vitro Study, Saudi Endodontic Journal (2022)
44. Histopathologic Evaluation of Subcutaneous Tissue Response to Three Endodontic Sealers in Rats., Journal of oral science (2011)
45. Evaluation of the Antibacterial Substantivity of Several Intra-Canal Agents, Australian Endodontic Journal (2006)