Isfahan University of Medical Sciences

Science Communicator Platform

Share By
Effects of Pomegranate Seed Extract and Ellagic Acid on Mir-16-5P and Mir-34A-5P Expression, Cell Cycle, and Apoptosis in Mcf-7 Cells Publisher



Sadeghi Z ; Sharifi M ; Malekzadeh M ; Ghaedamini S ; Hashemibeni B
Authors

Source: Research in Pharmaceutical Sciences Published:2026


Abstract

Background and purpose: The expression of Homo sapiens (has)-miR-16-5p and hsa-miR-34a-5p with anti-tumor effects is downregulated in cancer cells. To maintain these gene expressions in cancer cells, we evaluated the effects of pomegranate seed extract (PSE) and ellagic acid (EA) on hsa-miR-16-5p and hsa-miR- 34a-5p expression, cell cycle regulation, and apoptotic induction in the MCF-7 cells encapsulated in the alginate hydrogel, a 3D culture system. Experimental approach: MCF-7 cells were encapsulated in 3-D alginate scaffolds and cultured (experimental groups: EA, PSE, EA and PSE, and the control group). Cell viability (using MTT assay), cell apoptosis (via flow cytometry), the level of malondialdehyde (MDA), and the expression levels of hsa-miR-16-5p, hsa-miR- 34a-5p, B-cell lymphoma 2 (BCL-2), cyclin D1 (CCND1), and sirtuin 1 (SIRT1) were measured (via real-time PCR). Findings/Results: The combination of PSE and EA exhibited the greatest effects on MCF-7 cells. EA and PSE increased the expression of hsa-miR-16-5p and hsa-miR-34a-5p and decreased the expression of the SIRT1 and CCND1 genes. In addition, the antiapoptotic BCL-2 gene was downregulated in the experimental group. Both antioxidants significantly increased the population of MCF-7 cells in the G1 phase. Additionally, antioxidants reduce the level of MDA in cancer cells. Conclusion and implications: EA and PSE antioxidants increased hsa-miR-16-5p and hsa-miR-34a-5p expression, induced apoptosis, decreased cell proliferation, and stopped cancer cells in the G1 phase. Therefore, they can be considered promising compounds for helping the treatment of breast cancer. © 2026 Research in Pharmaceutical Sciences.
Related Docs
View other Related Docs
1. Anti-Tumor Effects of Ellagic Acid and Mesenchymal Stem Cell-Conditioned Medium on Breast Cancer Cells on the 3D Printed Pcl/Agarose Scaffolds, Journal of Drug Delivery Science and Technology (2024)
2. Britannin, a Sesquiterpene Lactone, Inhibits Proliferation and Induces Apoptosis Through the Mitochondrial Signaling Pathway in Human Breast Cancer Cells, Tumor Biology (2015)
3. Estrogen Receptor Beta (Erβ) May Act As Mediator in Apoptotic Induction of Grape Seed Extract (Gse), Asian Pacific Journal of Cancer Prevention (2019)
Experts (# of related papers)
View all Related Experts
Mustafa Ghanadian (4)
Mahmoud Aghaei (4)
Other Related Docs
4. Pomegranate Seed Extract Enhances the Inhibitory Effect of Adipose- Derived Mesenchymal Stem Cells on Breast Cancer Cell Line in Co-Culture Conditions, Research in Pharmaceutical Sciences (2022)
5. Persianolide-A, an Eudesmanolide-Type Sesquiterpene Lactone From Artemisia Kopetdaghensis, Induces Apoptosis by Regulating Erk Signaling Pathways, Research in Pharmaceutical Sciences (2024)
6. Investigating the Effect of Razurin Loaded Mesoporous Silica Nanoparticles Enwrapped With Chitosan-Folic Acid on Breast Tumor Regression in Balb/C Mice, International Journal of Biological Macromolecules (2025)
7. Centaurea Cyanus Extracted 13-O-Acetylsolstitialin a Decrease Bax/Bcl-2 Ratio and Expression of Cyclin D1/Cdk-4 to Induce Apoptosis and Cell Cycle Arrest in Mcf-7 and Mda-Mb-231 Breast Cancer Cell Lines, Journal of Cellular Biochemistry (2019)
8. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
9. Apoptosis-Inducing Effect of Spiroaminopyrimidine Analogue in Nb4 Leukemia Cells Via Down-Regulation of Birc5 Expression, Experimental Oncology (2019)
10. Multifunctional Estrone–Polyoxometalate–F16 Hybrid Conjugate: A Synergistic Targeting Approach Against Breast Cancer Cells, ACS Applied Bio Materials (2025)
11. Effect of 5'-Fluoro-2'-Deoxycytidine and Sodium Butyrate on the Gene Expression of the Intrinsic Apoptotic Pathway, P21, P27, and P53 Genes Expression, Cell Viability, and Apoptosis in Human Hepatocellular Carcinoma Cell Lines, Advanced Biomedical Research (2023)
12. Evaluation of Antitumor Effects of Aspirin and Lgk974 Drugs on Cellular Signaling Pathways, Cell Cycle and Apoptosis in Colorectal Cancer Cell Lines Compared to Oxaliplatin Drug, Fundamental and Clinical Pharmacology (2020)
13. The Growth of A549 Cell Line Is Inhibited by Pemetrexed Through Up-Regulation of Hsa-Mir-320A Expression, Advanced Biomedical Research (2024)
14. Combination of Salermide and Cholera Toxin B Induce Apoptosis in Mcf-7 But Not in Mrc-5 Cell Lines, International Journal of Preventive Medicine (2013)
15. Molecular Basis of Melatonin Protective Effects in Metastasis: A Novel Target of Melatonin, Biochimie (2022)
16. Dihydroartemisinin Enhances the Therapeutic Efficacy of Bh3 Mimetic Inhibitor in Acute Lymphoblastic Leukemia Cells Via Inhibition of Mcl-1, Asian Pacific Journal of Cancer Prevention (2024)
17. Disafynol: A Polyacetylene Dimer From Centaurea Schmidii Enhancing Breast Cancer Cell Apoptosis Via Oxidative and Er Stress Pathways, Naunyn-Schmiedeberg's Archives of Pharmacology (2025)
18. Cycloartane Triterpenoid From Euphorbia Macrostegia Modulates Er Stress Signaling Pathways to Induce Apoptosis in Mda-Mb231 and Mcf-7 Breast Cancer Cell Lines, Naunyn-Schmiedeberg's Archives of Pharmacology (2023)
19. Melatonin Affects the Expression of Microrna-21: A Mini-Review of Current Evidence, Pathology Research and Practice (2024)
20. Production and Evaluation of In-Vitro and In-Vivo Effects of P28-Il24, a Promising Anti-Breast Cancer Fusion Protein, International Journal of Peptide Research and Therapeutics (2021)