Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll)

Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll) Publisher Pubmed

Mousazadeh M¹ ; Palizban A¹ ; Salehi R² ; Salehi M²

Show Affiliations

1. Department of Clinical Biochemistry, School of Pharmacy and Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Drug Targeting Published:2007

Abstract

A promising strategy to carry genetic material to brain cells either in vitro or in vivo is using the LDL receptor (LDLr) on blood-brain barrier. LDLr naturally help to low density lipoproteins (LDLS) transporting across the BBB by endocytosis. Here we present the idea of using the LDLr-mediated pathway for transporting genetic material to brain cells. A tandem dimer Sequence of apoprotein-E (apoE) (141-150) conjugated to polylysine sequence was used as a novel DNA Delivery vector for transfecting of brain cells either in vitro or in vivo. DNA condensation occurs with this vector because electrostatic interaction between DNA and polylysine. The vector favors to protection of DNA from enzymatic degradation and also helps to DNA carrying in blood stream to reach BBB and transport it to brain cells and eventually help DNA expression in target cells. These results suggest a novel gene delivery vector for gene therapy of brain disease.

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Style	Citing Format
MLA	Mousazadeh M, et al.. "Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll)." Journal of Drug Targeting, vol. 15, no. 3, 2007, pp. 226-230.
APA	Mousazadeh M, Palizban A, Salehi R, Salehi M (2007). Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll). Journal of Drug Targeting, 15(3), 226-230.
Chicago	Mousazadeh M, Palizban A, Salehi R, Salehi M. "Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll)." Journal of Drug Targeting 15, no. 3 (2007): 226-230.
Harvard	Mousazadeh M et al. (2007) 'Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll)', Journal of Drug Targeting, 15(3), pp. 226-230.
Vancouver	Mousazadeh M, Palizban A, Salehi R, Salehi M. Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll). Journal of Drug Targeting. 2007;15(3):226-230.
BibTex	@article{ author = {Mousazadeh M and Palizban A and Salehi R and Salehi M}, title = {Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll)}, journal = {Journal of Drug Targeting}, volume = {15}, number = {3}, pages = {226-230}, year = {2007} }
RIS	TY - JOUR AU - Mousazadeh M AU - Palizban A AU - Salehi R AU - Salehi M TI - Gene Delivery to Brain Cells With Apoprotein E Derived Peptide Conjugated to Polylysine (Apoedp-Pll) JO - Journal of Drug Targeting VL - 15 IS - 3 SP - 226 EP - 230 PY - 2007 ER -

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