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Gene Delivery Using Lipoplexes and Polyplexes: Principles, Limitations and Solutions Publisher Pubmed



Hayat SMG1 ; Farahani N2 ; Safdarian E3 ; Roointan A4, 5 ; Sahebkar A6, 7, 8
Authors
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Authors Affiliations
  1. 1. Department of Genetics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  2. 2. Department of Genetics and Molecular Biology, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Legal Medicine Center, Legal Medicine Organization, Tehran, Iran
  4. 4. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz, Iran
  5. 5. Department of Medical Biotechnology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
  6. 6. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  7. 7. Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  8. 8. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Critical Reviews in Eukaryotic Gene Expression Published:2019


Abstract

Gene therapy has attracted considerable attention for the treatment of genetic and acquired diseases. Successful gene therapy occurs when the therapeutic genes penetrate targeted cells and become available to the intracellular active site. Currently, a promising approach in gene delivery is the use of nonviral gene delivery vectors that lack immunogenicity but have low toxicity and potential tissue specificity. The widely used, existing nonviral gene vectors are cationic lipids and polymers that can pass across extracellular and intracellular barriers. However, the toxicity of these vectors is a barrier to their use. Currently, the disadvantages of nonviral vectors have been minimized by several modifications. The main purpose of this review is to describe the pros and cons of gene delivery using cationic lipids and polymers. r Pr. © 2019 Begell House, Inc.
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