Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Potential Anti-Cancer Effects of Hibernating Common Carp (Cyprinus Carpio) Plasma on B16-F10 Murine Melanoma: In Vitro and in Vivo Studies Publisher Pubmed



Amini E1 ; Rahgozar S1 ; Golpich M2 ; Kefayat A3 ; Fesharaki M4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
  2. 2. Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
  3. 3. Department of Oncology, Cancer Prevention Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Cell Sciences Research Center Medical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: International Journal of Biological Macromolecules Published:2023


Abstract

Melanoma is the major type of skin cancer, which its treatment is still a challenge in the world. In recent years, interest in hibernation-based therapeutic approaches for various biomedical applications has been increased. Many studies indicated that some factors in the blood plasma of hibernating animals such as alpha-2-macroglobulin (A2M) cause anti-proliferative effects. Considering that, the present study was conducted to investigate the anti-cancer effects of hibernating common carp plasma (HCCP) on murine melanoma (B16-F10) in vitro and in vivo. The effect of HCCP on cell viability, migration, apoptosis rate, and cell cycle distribution of B16-F10 cells, tumor growth, and rate of survival were evaluated. To investigate the role of A2M in the anti-cancer effects of HCCP, the gene of interest and proteins in HCCP and non-hibernating common carp plasma (NHCCP) were evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay as well as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry analysis. Based on our findings, HCCP significantly decreased B16-F10 cell viability. Moreover, HCCP caused morphological alternations, inhibition of migration, induction of apoptosis, and significantly induced the cell cycle arrest at the G2/M phase. In addition, A2M level was significantly increased in HCCP compared with NHCCP. Taken together, our findings suggested that HCCP had the potential to be a promising novel therapeutic target for cancer treatment because of its anti-cancer properties. © 2023 Elsevier B.V.
Related Docs
View other Related Docs
1. In Vitro and in Vivo Anti-Cancer Effects of Hibernating Common Carp (Cyprinus Carpio) Plasma on Metastatic Triple-Negative Breast Cancer, Scientific Reports (2022)
2. Immunoprotective Effect of an in Silico Designed Multiepitope Cancer Vaccine With Boris Cancer-Testis Antigen Target in a Murine Mammary Carcinoma Model, Scientific Reports (2021)
3. Synthesis and Characterization of Cellulose Nanofibers/Chitosan/Cinnamon Extract Wound Dressing With Significant Antibacterial and Wound Healing Properties, Journal of the Iranian Chemical Society (2022)
4. Targeting Ferroptosis As a Cell Death Pathway in Melanoma: From Molecular Mechanisms to Skin Cancer Treatment, International Immunopharmacology (2023)
5. The Role of Heme Oxygenase -1 System in Melanoma Tumor Growth of C57bl/6 Mice, Journal of Zanjan University of Medical Sciences and Health Services (2014)
6. Evaluation of Ultrasound-Mediated Chemotherapy Treatment Using Dacarbazine on B16f10 Melanoma Cell Line, Journal of Isfahan Medical School (2022)
7. Biodegradable and Biocompatible Subcutaneous Implants Consisted of Ph-Sensitive Mebendazole-Loaded/Folic Acid-Targeted Chitosan Nanoparticles for Murine Triple-Negative Breast Cancer Treatment, Journal of Nanobiotechnology (2022)
8. Standardized Punica Granatum Pericarp Extract, Suppresses Tumor Proliferation and Angiogenesis in a Mouse Model of Melanoma: Possible Involvement of Pparα and Pparγ Pathways, Iranian Journal of Pharmaceutical Research (2019)
Experts (# of related papers)
View all Related Experts
Mohammad Rafienia (3)
Shaghayegh Haghjooy Javanmard (3)
Other Related Docs
9. Polyurethane-Nanolignin Composite Foam Coated With Propolis As a Platform for Wound Dressing: Synthesis and Characterization, Polymers (2021)
10. Utilizing 808 Nm Laser for Sensitizing of Melanoma Tumors to Megavoltage Radiation Therapy, Lasers in Medical Science (2020)
11. Anti-Cancer Immunoprotective Effects of Immunization With Hydatid Cyst Wall Antigens in a Non-Immunogenic and Metastatic Triple-Negative Murine Mammary Carcinoma Model, International Immunopharmacology (2021)
12. The Effects of Alk5 Inhibition and Simultaneous Inhibition or Activation of Hif-1Α in Melanoma Tumor Growth and Angiogenesis, Tumor Biology (2023)