Beneficial Effects of Se/Zn Co-Supplementation on Body Weight and Adipose Tissue Inflammation in High-Fat Diet-Induced Obese Rats Publisher



Hasani M¹ ; Saidpour A² ; Irandoost P¹ ; Golab F³ ; Khazdouz M¹ ; Qorbani M^{4, 5} ; Agh F¹ ; Mohammad Sharifi A⁶ ; Vafa M¹ Show Affiliations
Source: Food Science and Nutrition Published:2021

Abstract

This research investigated the effect of co-supplementation of selenium with zinc on weight control and the inflammatory and oxidative status in relation to obesity. Male Wistar rats (N = 32) were randomly divided into four groups after induction of obesity model: 1) “Zn” was supplemented with zinc sulfate (15 mg/kg BW), 2) “Se” supplemented with selenium as sodium selenate (0.5 mg/kg BW), 3) “Zn + Se” which received Zn (15 mg/kg BW) + Se (0.5 mg/kg BW), and 4) “HFD” as the control group. The intervention was done for eight weeks. At the end of treatment, serum and tissue level of Zn, Se, SOD, GSH-Px, MDA, leptin, TNF-α, and IL-6 was evaluated. Weight and food intake were significantly reduced in the Se group(p <.001), while in the Zn group, weight gain due to obesity was prevented compared to the control group (p =.48). There was a significant and stronger increase in SOD, GSH-Px levels and a remarkable decrease in MDA, leptin, TNF-α, and IL-6 in the group receiving the combination of two supplements than either alone(p <.001). Leptin had a positive correlation with inflammatory factors and lipid peroxidation marker and showed an inverse relationship with Zn and Se levels and anti-oxidative enzymes(p <.05). The analysis showed the mediating role of leptin in the effects of zinc. Co-supplementation of selenium and zinc may have a synergistic effect in reduction of oxidative and inflammatory markers. Regarding the effect of zinc on inflammatory factors and lipid peroxidation, leptin can play a mediating role. © 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC