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Proteome Analysis of Propolis Deciphering the Origin and Function of Its Proteins Publisher



Shahali Y1 ; Kler S2 ; Revets D2 ; Planchon S3 ; Leclercq CC3 ; Renaut J3 ; Shoormasti RS4 ; Pourpak Z4 ; Ollert M2, 5 ; Hilger C2
Authors
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Authors Affiliations
  1. 1. University Hospital of Besancon, Besancon, France
  2. 2. Department of Infection and Immunity, Luxembourg Institute of Health (LIH), Esch-sur-Alzette, Luxembourg
  3. 3. Department of Environmental Research and Innovation, Luxembourg Institute of Science and Technology (LIST), Esch-sur-Alzette, Luxembourg
  4. 4. Immunology, Asthma and Allergy Researche Intiture, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Dermatology and Allergy Center, Odense Research Center for Anaphylaxis, University of Southern Denmark, Odense, Denmark

Source: Journal of Food Composition and Analysis Published:2024


Abstract

Propolis is a resinous honeybee product, rich in polyphenolic compounds and with high economic value. Although extensive studies regarding the chemical composition of different propolis extracts have been carried out, the propolis proteome remained unknown. The present study aimed at characterizing the proteome of two geographically-distinct propolis originating from Belgium and Iran. Propolis extracts were analyzed by sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) followed by Matrix Assisted Laser Desorption Ionization – Time of Flight Mass Spectrometry (MALDI-TOF MS) and Liquid chromatography-electrospray ionization MS (LC-ESI-MS) analyses. Our comprehensive proteomic analysis led to the identification of honeybee venom and royal jelly proteins, and numerous plant-defense proteins in propolis, extending our understanding of the antimicrobial properties of this complex natural substance. The proteome of both propolis was mainly made up of proteins belonging to the poplar tree. Pathogenesis-related (PR) proteins, poplar PR-2 (β-1,3-glucanases) and PR-8 (acidic endochitinases) were found to be the major components of both propolis extracts, while the major bee venom allergen (Api m 1) and royal jelly proteins (MJRP 1, 2, 3 and 5) were identified in the propolis from Belgium. Overall, our proteome analyses revealed that propolis extracts from Belgium and Iran shared a core protein composition originating from the poplar proteome. The majority of identified proteins are involved in the plant defense against pathogens, most belonging to well-known pathogenesis-related families. Proteomics could thus represent a viable approach for origin authentication and allergen identification in poplar-type propolis. © 2023 Elsevier Inc.
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