Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro

Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro Publisher Pubmed

Alkafaji HA¹ ; Raji A¹ ; Rahman HS² ; Zekiy AO³ ; Adili A⁴ ; Jalili M⁵ ; Hojjatipour T⁶ ; Cidarregui A⁷ ; Shomali N^{5, 8} ; Tarzi S⁵ ; Tamjidifar R⁵ ; Heshmati R⁵ ; Marofi F⁸ ; Akbari M⁵ Show All Authors

Alkafaji HA¹
Raji A¹
Rahman HS²
Zekiy AO³
Adili A⁴
Jalili M⁵
Hojjatipour T⁶
Cidarregui A⁷
Shomali N^{5, 8}
Tarzi S⁵
Tamjidifar R⁵
Heshmati R⁵
Marofi F⁸
Akbari M⁵
Hasanzadeh A⁸
Deljavanghodrati M⁵
Jarahian M⁹
Sandoghchian Shotorbani S^{5, 8}

Show Affiliations

1. College of medicine, University of Babylon, Babylon, Iraq
2. Department of Physiology, College of Medicine, University of Suleimanyah, Suleimanyah, Iraq
3. Sechenov First Moscow State Medical University, Moscow, Russian Federation
4. Department of Oncology, Tabriz University of Medical Sciences, Tabriz, Iran
5. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6. Department of Hematology and Blood Transfusion, Students Research Centre, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
7. Targeted Tumor Vaccines Unit, German Cancer Research Center (DKFZ), Heidelberg, Germany
8. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran
9. Toxicology and Chemotherapy Unit (G401), German Cancer Research Center, Heidelberg, Germany

Source: Journal of Cellular and Molecular Medicine Published:2021

Abstract

Melanoma is a kind of skin cancer that is begun by the alteration of melanocytes. miRNAs are small non-coding RNA molecules that regulate a variety of biological processes. KISS1, the metastasis-suppressor gene, encodes kisspeptins which inhibits migration and proliferation of cancers. This study was aimed to determine the role of Let-7i and KISS1 in melanoma cell migration and proliferation. At first, the expression of Let-7i and KISS1 was determined in patients with melanoma. In the in vitro part of the study, Let-7i mimics were transfected and the impact of its restoration on target gene expression, proliferation, migration and apoptosis of SK-MEL-3 melanoma cell line was assessed by real-time PCR and Western blotting, MTT assay, wound-healing assay and flow cytometry, respectively. Besides, KISS1 inhibitor siRNA alone and along with Let-7i was transfected to determine their probable correlation. The results revealed that either Let-7i or KISS1 were down-regulated in patients with melanoma. The results obtained from the in vitro part of the study revealed that restoration of Let-7i reduced the expression of metastasis- and proliferation-related target genes. Moreover, it was revealed that up-regulation of Let-7i attenuated migration and proliferation capability of SK-MEL-3 cells. Besides, it was demonstrated that Let-7i restoration induced apoptosis in melanoma cells. More importantly, the KISS1 inhibitor caused a prominent cell migration and proliferation, attenuated by Let-7i re-expression. To sum up, the present study revealed the impressive role of Let-7i restoration along with its correlation with KISS1 on melanoma carcinogenicity which may be applicable in future in vivo studies. © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

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Style	Citing Format
MLA	Alkafaji HA, et al.. "Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro." Journal of Cellular and Molecular Medicine, vol. 25, no. 14, 2021, pp. 6864-6873.
APA	Alkafaji HA, Raji A, Rahman HS, Zekiy AO, Adili A, Jalili M, Hojjatipour T, Cidarregui A, Shomali N, Tarzi S, Tamjidifar R, Heshmati R, Marofi F, Akbari M, Hasanzadeh A, Deljavanghodrati M, Jarahian M, Sandoghchian Shotorbani S (2021). Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro. Journal of Cellular and Molecular Medicine, 25(14), 6864-6873.
Chicago	Alkafaji HA, Raji A, Rahman HS, Zekiy AO, Adili A, Jalili M, Hojjatipour T, et al.. "Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro." Journal of Cellular and Molecular Medicine 25, no. 14 (2021): 6864-6873.
Harvard	Alkafaji HA et al. (2021) 'Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro', Journal of Cellular and Molecular Medicine, 25(14), pp. 6864-6873.
Vancouver	Alkafaji HA, Raji A, Rahman HS, Zekiy AO, Adili A, Jalili M, et al.. Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro. Journal of Cellular and Molecular Medicine. 2021;25(14):6864-6873.
BibTex	@article{ author = {Alkafaji HA and Raji A and Rahman HS and Zekiy AO and Adili A and Jalili M and Hojjatipour T and Cidarregui A and Shomali N and Tarzi S and Tamjidifar R and Heshmati R and Marofi F and Akbari M and Hasanzadeh A and Deljavanghodrati M and Jarahian M and Sandoghchian Shotorbani S}, title = {Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro}, journal = {Journal of Cellular and Molecular Medicine}, volume = {25}, number = {14}, pages = {6864-6873}, year = {2021} }
RIS	TY - JOUR AU - Alkafaji HA AU - Raji A AU - Rahman HS AU - Zekiy AO AU - Adili A AU - Jalili M AU - Hojjatipour T AU - Cidarregui A AU - Shomali N AU - Tarzi S AU - Tamjidifar R AU - Heshmati R AU - Marofi F AU - Akbari M AU - Hasanzadeh A AU - Deljavanghodrati M AU - Jarahian M AU - Sandoghchian Shotorbani S TI - Up-Regulation of Kiss1 As a Novel Target of Let-7I in Melanoma Serves As a Potential Suppressor of Migration and Proliferation in Vitro JO - Journal of Cellular and Molecular Medicine VL - 25 IS - 14 SP - 6864 EP - 6873 PY - 2021 ER -

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