Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Aqueous Nigella Sativa Extract on the Functional Parameters of Post-Thaw Human Spermatozoa During Vitrification Publisher



Nasiri Z1 ; Ghorbani F2 ; Seify M3, 4 ; Sharbati A5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Reproductive Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  4. 4. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  5. 5. Department of Medical Surgical Nursing, Faculty of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran

Source: Clinical and Experimental Reproductive Medicine Published:2022


Abstract

Objective: Sperm vitrification leads to the production of reactive oxygen species (ROS) that can damage the functional parameters of sperm. The present study aimed to investigate the antioxidant effect of Nigella sativa extract on motility, plasma membrane function, mitochondrial membrane potential (MMP), DNA damage, and intracellular ROS production. Methods: A total of 20 sperm samples were used. Samples were divided into six experimental groups, including groups with aqueous extract from N. sativa seeds at concentrations of 1% to 6%, a cryopreserved control group, and a fresh control group. Results: Statistical analysis showed significantly higher total sperm motility at concentrations of 3% to 6% than in the vitrified semen control group. Additionally, progressive motility and all motion characteristics at all concentrations were significantly higher than in the vitrified semen control group. The presence of N. sativa seed extract also improved the quality of the sperm parameters assayed in all experimental groups (1%–6%; intracellular ROS production, DNA damage, MMP, and sperm membrane function) compared to the control group. Conclusion: Higher concentrations of N. sativa led to improvements in all sperm parameters and sperm quality. These findings indicate that N. sativa seed extract is effective for improving the quality of sperm after vitrification. © 2022. THE KOREAN SOCIETY FOR REPRODUCTIVE MEDICINE
Related Docs
1. The Antioxidant Roles of L-Carnitine and N-Acetyl Cysteine Against Oxidative Stress on Human Sperm Functional Parameters During Vitrification, Clinical and Experimental Reproductive Medicine (2021)
2. The Effects of Purslane (Portulaca Oleracea) and Fennel (Foeniculum Vulgare Mill) Hydroalcoholic Extracts on the Functional Parameters of Human Spermatozoa After Vitrification, Clinical and Experimental Reproductive Medicine (2023)
3. The Role of Antioxidants in Sperm Freezing: A Review, Cell and Tissue Banking (2016)
4. Effect of Sulforaphane on Apoptosis, Reactive Oxygen Species and Lipids Peroxidation of Human Sperm During Cryopreservation, Cryobiology (2021)
5. Nigella Sativa L., Therapeutic Medicinal Plants in Traditional Persian Medicine (2024)
6. Supplementation of Freezing and Thawing Media With Brain-Derived Neurotrophic Factor Protects Human Sperm From Freeze-Thaw-Induced Damage, Fertility and Sterility (2016)
7. An Evidence-Based Approach to Medicinal Plants for the Treatment of Sperm Abnormalities in Traditional Persian Medicine, Andrologia (2016)
8. Effects of Nigella Sativa on Glycemic Control, Lipid Profiles, and Biomarkers of Inflammatory and Oxidative Stress: A Systematic Review and Meta-Analysis of Randomized Controlled Clinical Trials, Phytotherapy Research (2020)
Experts (# of related papers)
View all Related Experts
Fardin Amidi (3)
Maryam Shabani Nashtaee (2)