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Does Myoinositol Supplement Improve Sperm Parameters and Dna Integrity in Patients With Oligoasthenoteratozoospermia After the Freezing–Thawing Process? Publisher Pubmed



Abdolsamadi M1 ; Mohammadi F2 ; Nashtaei MS3 ; Teimouri M4 ; Sardar R2 ; Dayani M5 ; Haghighi M2 ; Ghasemi S6 ; Vatannejad A7, 8 ; Zandieh Z2, 6
Authors
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Authors Affiliations
  1. 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Anatomical Sciences, Iran University of Medical Science, Tehran, Iran
  3. 3. Department of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Radiation Oncology Department, Emam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran
  6. 6. Shahid Akbarabadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Science, Tehran, Iran
  7. 7. Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  8. 8. Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Cell and Tissue Banking Published:2020


Abstract

Sperm cryopreservation is a routine method in andrology and IVF laboratory. However, the sperm quality and its fertilizing capacity have been decreased during this process. The purpose of this experiment was to determine the role of myoinositol as a supplement in amelioration of total and progressive sperm motility, DNA fragmentation, total antioxidant capacity (TAC), reactive oxygen species (ROS), and lipid peroxidation after the freezing–thawing process on patients with oligoasthenoteratozoospermia (OAT) syndrome. Semen samples obtained from 40 patients were divided into two aliquots and freezed with simple and 2 mg/mL myoinositol (MYO) supplemented freezing media. All samples were thawed and assessed after one month. Semen parameters were analyzed in terms of the motility by CASA, the level of total ROS by fluorimetry, TAC and MDA by colorimetric assay and finally DNA fragmentation by TUNEL assay. Our results clearly showed that MYO could improve total (37.46 vs. 12.91, p < 0.001) and progressive motility (21.92 vs. 6.49, p < 0.001) in experimental group compared to control group. A higher TAC level was observed in the MYO treated group in comparison to control group (1.11 vs. 0.91, p = 0.05). While MYO supplementation could not be effective on ROS level, it reduced DNA fragmentation of sperm after freeze–thaw process (p = 0.01). Therefore, MYO could be a good supplement for sperm freezing to reduce the detrimental effects of freezing process especially on DNA integrity, which is an important factor in the success of ART, in OAT suffered patients. © 2019, Springer Nature B.V.
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