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Protective Effects of Astaxanthin on Post-Thaw Sperm Quality in Normozoospermic Men Publisher



Mohammadibardbori A1 ; Shadboorestan A2 ; Salehi E3 ; Rahimi MA4 ; Kargarabarghouei E5 ; Amidi F6 ; Shamseddin J7 ; Omidi M8, 9
Authors
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Authors Affiliations
  1. 1. Department of Pharmacology and Toxicology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Department of Gynecology, School of Medicine, Fertility and Infertility Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  4. 4. Student Research Committee, Hormozgan University of Medical Sciences, Bandar Abbas Hums.ac.ir, Iran
  5. 5. Department of Anatomy, School of Medicine, Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  6. 6. Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Shahid Chamran Boulevard, Bandar Abbas, Iran
  8. 8. Food Health Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  9. 9. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

Source: Andrologia Published:2024


Abstract

Fighting against free radical accumulation during the cryopreservation process with the help of exogenous antioxidants has become an effective strategy to augment post-thaw sperm quality. The present study explored the effects of various concentrations of astaxanthin, as a potent antioxidant, on human sperm parameters during the freeze-thaw process. Twenty-five normozoospermic specimens were included in this prospective study. Each sample was divided into five equal parts: fresh group, and frozen-thawed groups including 0, 1, 10, and 50 μM of astaxanthin. The prepared spermatozoa were cryopreserved by rapid freezing technique. Precryopreservation and post-thaw sperm motility parameters, sperm morphology, vitality, DNA fragmentation, reactive oxygen species (ROS) levels, and mitochondrial membrane potential (MMP) were investigated. All sperm parameters after thawing significantly decreased compared to before freezing. Treatment of spermatozoa with 50 μM of astaxanthin significantly increased their total and progressive motility, viability, DNA integrity, and MMP and decreased their intracellular ROS levels compared with the control group. In total, 10 μM of astaxanthin significantly improved total and progressive motility, DNA integrity, MMP, and decreased ROS levels, whereas, in the 1 μM group, there were significant differences only in ROS levels. As a result, we found that astaxanthin can improve sperm quality after freezing/thawing and decrease the detrimental effects of this process on sperm parameters. © 2024 Afshin Mohammadi-Bardbori et al.
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