The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates

The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates Publisher Pubmed

Imani Fooladi AA¹ ; Ashrafi E² ; Tazandareh SG³ ; Koosha RZ⁴ ; Rad HS¹ ; Amin M⁵ ; Soori M³ ; Larki RA⁶ ; Choopani A⁷ ; Hosseini HM¹

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1. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2. Microbiology Department, Islamic Azad University of Arak Branch, Arak, Iran
3. Microbiology Department, Islamic Azad University of Zanjan Branch, Zanjan, Iran
4. Biology Department, Islamic Azad University of Jahrom Branch, Jahrom, Iran
5. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
6. Microbiology Department, Islamic Azad University of Tonekabon Branch, Jahrom, Iran
7. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Microbial Pathogenesis Published:2015

Abstract

Staphylococcus aureus (. S.aureus) is considered as a notorious nosocomial pathogen among hospitalized patients and community-dwelling subjects. Its increasing morbidity and mortality is believed to be due to antibiotic resistance. However, the data concerning molecular properties of infecting strains are few.In this study, a total of 192 S.aureus strains, including 88 (45.8%) meticillin-sensitive S. aureus (MSSA) and 104 (54.2%) meticillin-resistant S. aureus (MRSA) were recovered from clinical samples. The prevalence of subtypes containing staphylococcal cassette chromosome mec (SSC. mec), staphylococcal enterotoxins (SEs), toxic shock syndrome toxin (TSST) and exfoliative toxin was assessed by PCR. Antibiotic susceptibility pattern and vancomycin resistance of each isolate were evaluated by disk diffusion method and micro-dilution method, respectively.9 (2.3%) strains required MIC>2mg/l of vancomycin, which significantly increased among multi drug resistant (MDR), MRSA and SCC. mec type III strains (p<0.05). 171 (89%), 140 (72.91%), 7 (3.6), 78 (48.6%), 5 (2.6%), 151 (78.64%), 129 (67.18%), 178 (92.7%) and 15 (7.8%) of 192 isolates harbored mecA, entA, entB, entC, entD, entE, eta, etb and tsst-1 genes, respectively. 31 (16.14%), 5 (2.6%), 95 (49.48%) and 7 (3.64%) of 192 isolates carried SCC. mec type I, II, III and IV, respectively. We found a significantly higher rate of MRSA and resistance to all tested antibiotics, except to penicillin G, kanamycin and linezolide among the SCC. mec type III class (p<0.05).According to our findings, MSSA isolates should be taken as seriously as MRSA strains due to the potential presence of broad spectrum virulence factor genes. © 2015 Elsevier Ltd.

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Style	Citing Format
MLA	Imani Fooladi AA, et al.. "The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates." Microbial Pathogenesis, vol. 81, no. , 2015, pp. 60-66.
APA	Imani Fooladi AA, Ashrafi E, Tazandareh SG, Koosha RZ, Rad HS, Amin M, Soori M, Larki RA, Choopani A, Hosseini HM (2015). The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates. Microbial Pathogenesis, 81(), 60-66.
Chicago	Imani Fooladi AA, Ashrafi E, Tazandareh SG, Koosha RZ, Rad HS, Amin M, Soori M, Larki RA, Choopani A, Hosseini HM. "The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates." Microbial Pathogenesis 81, no. (2015): 60-66.
Harvard	Imani Fooladi AA et al. (2015) 'The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates', Microbial Pathogenesis, 81(), pp. 60-66.
Vancouver	Imani Fooladi AA, Ashrafi E, Tazandareh SG, Koosha RZ, Rad HS, Amin M, et al.. The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates. Microbial Pathogenesis. 2015;81():60-66.
BibTex	@article{ author = {Imani Fooladi AA and Ashrafi E and Tazandareh SG and Koosha RZ and Rad HS and Amin M and Soori M and Larki RA and Choopani A and Hosseini HM}, title = {The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates}, journal = {Microbial Pathogenesis}, volume = {81}, number = {}, pages = {60-66}, year = {2015} }
RIS	TY - JOUR AU - Imani Fooladi AA AU - Ashrafi E AU - Tazandareh SG AU - Koosha RZ AU - Rad HS AU - Amin M AU - Soori M AU - Larki RA AU - Choopani A AU - Hosseini HM TI - The Distribution of Pathogenic and Toxigenic Genes Among Mrsa and Mssa Clinical Isolates JO - Microbial Pathogenesis VL - 81 IS - SP - 60 EP - 66 PY - 2015 ER -

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