Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique

Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique Publisher

Ghobadi MZ^{1, 2} ; Mozhgani SH^{3, 4} ; Hakimian F¹ ; Norouzi M^{2, 4} ; Rezaee SA⁵ ; Ghourchian H¹

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1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
2. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
4. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
5. Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Heliyon Published:2018

Abstract

Detecting fluorescence changes due to energy transfer between a quencher and fluorophore is a common method used for the fluorescence-based biosensors. In the present report, a new biosensor for long segment detection of the human T cell-lymphotropic virus 1 genome was constructed based on the fluorescence quenching of graphene oxide by gold nanoparticles. The fluorescence signal of unmodified graphene oxide was measured before and after hybridization of target and probes functionalized with gold nanoparticles. The limit of detection of the biosensor was determined to be around 10 pg/mL. The specific design for long segment of target assures the selectivity of biosensor. Our results proposed that further development may be useful to detect other viruses. © 2018

2. A Genosensor for Detection of Htlv-I Based on Photoluminescence Quenching of Fluorescent Carbon Dots in Presence of Iron Magnetic Nanoparticle-Capped Au, Scientific Reports (2018)

3. Recent Advances in Gold Nanoparticles-Based Biosensors for Tuberculosis Determination, Talanta (2024)

4. Identification of Joint Gene Players Implicated in the Pathogenesis of Htlv-1 and Blv Through a Comprehensive System Biology Analysis, Microbial Pathogenesis (2021)

5. Signaling Factors Potentially Associated to the Pathogenesis of Adult T-Cell Leukemia /Lymphoma: A Network-Analysis and Novel Findings Assessment, Virus Research (2022)

6. An Insight to Htlv-1-Associated Myelopathy/Tropical Spastic Paraparesis (Ham/Tsp) Pathogenesis; Evidence From High-Throughput Data Integration and Meta-Analysis, Retrovirology (2019)

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Mehdi Norouzi (4)

Hamid Farajifard (2)

Style	Citing Format
MLA	Ghobadi MZ, et al.. "Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique." Heliyon, vol. 4, no. 12, 2018, pp. -.
APA	Ghobadi MZ, Mozhgani SH, Hakimian F, Norouzi M, Rezaee SA, Ghourchian H (2018). Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique. Heliyon, 4(12), -.
Chicago	Ghobadi MZ, Mozhgani SH, Hakimian F, Norouzi M, Rezaee SA, Ghourchian H. "Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique." Heliyon 4, no. 12 (2018): -.
Harvard	Ghobadi MZ et al. (2018) 'Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique', Heliyon, 4(12), pp. -.
Vancouver	Ghobadi MZ, Mozhgani SH, Hakimian F, Norouzi M, Rezaee SA, Ghourchian H. Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique. Heliyon. 2018;4(12):-.
BibTex	@article{ author = {Ghobadi MZ and Mozhgani SH and Hakimian F and Norouzi M and Rezaee SA and Ghourchian H}, title = {Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique}, journal = {Heliyon}, volume = {4}, number = {12}, pages = {-}, year = {2018} }
RIS	TY - JOUR AU - Ghobadi MZ AU - Mozhgani SH AU - Hakimian F AU - Norouzi M AU - Rezaee SA AU - Ghourchian H TI - Long Segment Detection of Htlv-1 Genome Based on the Fluorescence Quenching Technique JO - Heliyon VL - 4 IS - 12 SP - EP - PY - 2018 ER -

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