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Electrochemiluminescence Paper-Based Screen-Printed Electrode for Hba1c Detection Using Two-Dimensional Zirconium Metal-Organic Framework/Fe3o4 Nanosheet Composites Decorated With Au Nanoclusters Publisher Pubmed



Ahmadi A1, 2 ; Khoshfetrat SM3 ; Kabiri S1, 2 ; Dorraji PS4 ; Larijani B2 ; Omidfar K1, 2
Authors
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Authors Affiliations
  1. 1. Biosensor Research Center, Endocrinology and Metabolism Molecular–Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Chemistry Department, Faculty of Science, Ayatollah Boroujerdi University, Borujerd, Iran
  4. 4. Department of Chemistry, Faculty of Physics and Chemistry, Alzahra University, Tehran, Iran

Source: Microchimica Acta Published:2021


Abstract

Glycated hemoglobin (HbA1c) is one of the most popular biomarkers which can be utilized for the diagnosis and control of diabetes in clinical practice. In this study, a sandwich paper-based electrochemiluminescence (ECL) biosensor has been developed using the zirconium metal-organic framework/Fe3O4(trimethyl chitosan)/gold nanocluster (Zr-MOF/Fe3O4(TMC)/AuNCs) nanocomposite as tracing tag to label anti-HbA1c monoclonal antibody and reduced graphene oxide (rGO) as immobilization platform of sensing element. The screen-printed electrodes (SPEs) were constructed and modified by sputtering a thick layer of gold on the paper substrate, followed by electrochemical reduction of aminophenylboronic acid (APBA)–functionalized GO to rGO/APBA, respectively. Different types of surface analysis methods were applied to characterize the Zr-MOF/Fe3O4(TMC)/AuNCs nanomaterials fabricated. Finally, antibody-labeled Zr-MOF/Fe3O4(TMC)/AuNCs nanocomposites were subjected to HbA1c in the sample and on the paper-based SPE. Quantitative measurement of HbA1c was performed using ECL and cyclic voltammetry (CV) over a potential range of − 0.2 to 1.7 V vs gold reference electrode with a sweep rate of 0.2 V.s−1 in the presence of triethylamine as a co-reactant after sandwiching the HbA1c target between antibody and APBA on the sensing area. This immunosensor demonstrated the desirable assay performance for HbA1c with a wide response range from 2 to 18% and a low detection limit (0.072%). This new strategy provides an effective method for high-performance bioanalysis and opens avenues for the development of high-sensitive and user-friendly device. [Figure not available: see fulltext.] © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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