Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
Targeted Dna Delivery to Cancer Cells Using a Biotinylated Chitosan Carrier Publisher Pubmed



Darvishi MH1 ; Nomani A2 ; Hashemzadeh H3 ; Amini M4 ; Shokrgozar MA5 ; Dinarvand R6, 7
Authors

Source: Biotechnology and Applied Biochemistry Published:2017


Abstract

A novel biotinylated chitosan-graft-polyethyleneimine (Bio-Chi-g-PEI) copolymer was synthesized and evaluated as a nonviral gene delivery carrier for improvement of the transfection efficiency, endosomal escape, and targeted gene delivery of a plasmid encoding green fluorescent protein N1 (pEGFP-N1) into two different biotin-overexpressing cell lines including HeLa and OVCAR-3 cells. The structure of the obtained copolymers was confirmed by 1H nuclear magnetic resonance (1H NMR) and Fourier transform infrared spectroscopy. Physicochemical properties of the Bio-Chi-g-PEI/plasmid DNA (pDNA) complexes such as complex stability, size, zeta potential, and their morphology were investigated at various weight ratios of copolymer to pDNA. Bio-Chi-g-PEI copolymers could effectively condense pDNA into small particles with average diameters less than 164 nm and the zeta potential of +34.8 mV at the N/P ratio of 40/1. As revealed by flow cytometry, Bio-Chi-g-PEI/pDNA complexes had lower cytotoxicity than that of PEI 25 kDa/pDNA complexes in both cell lines. In vitro experiments revealed that the Bio-Chi-gPEI/pDNA complexes not only had much lower cytotoxicity, but also displayed higher transfection efficiency than that of PEI 25kDa/pDNA complexes. High percentage of cancer cells was successfully transfected by Bio-Chi-g-PEI/pDNA and properly expressed GFP protein. This study indicates that this copolymer complex can be a promising gene delivery carrier. © 2016 International Union of Biochemistry and Molecular Biology, Inc.
Related Docs
View other Related Docs
1. Preparation and Optimization of Chitosan/Pdna Nanoparticles Using Electrospray, Proceedings of the National Academy of Sciences India Section B - Biological Sciences (2019)
2. Preparation of Mesalamine Nanoparticles Using a Novel Polyurethane-Chitosan Graft Copolymer, Pharmaceutical Nanotechnology (2017)
3. Novel Chitosan Based Nanoparticles As Gene Delivery Systems to Cancerous and Noncancerous Cells, International Journal of Pharmaceutics (2019)
Experts (# of related papers)
View all Related Experts
Fatemeh Atyabi (5)
Farid Dorkoosh (4)
Other Related Docs
4. Chitosan-Based Smart Stimuli-Responsive Nanoparticles for Gene Delivery and Gene Therapy: Recent Progresses on Cancer Therapy, International Journal of Biological Macromolecules (2024)
5. Optimization of Chitosan Nanoparticles As an Anti-Hiv Sirna Delivery Vehicle, International Journal of Biological Macromolecules (2019)
6. Chitosan As a Machine for Biomolecule Delivery: A Review, Carbohydrate Polymers (2021)
7. Smart Physicochemical-Triggered Chitosan-Based Nanogels for Sirna Delivery and Gene Therapy: A Focus on Emerging Strategies and Paradigms for Cancer Therapy, Current Medicinal Chemistry (2025)
8. Potential of Chitosan/Alginate Nanoparticles As a Non-Viral Vector for Gene Delivery: Formulation and Optimization Using D-Optimal Design, Materials Science and Engineering C (2021)
9. Evaluation of Antimicrobial Activity of Thiolated Methylated N-(4-N,N-Dimethylaminobenzyl) Chitosan As a New Derivative of Chitosan, Letters in Applied Microbiology (2022)
10. Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System, Cytology and Genetics (2015)
11. Nano Polyelectrolyte Complexes of Carboxymethyl Dextran and Chitosan to Improve Chitosan-Mediated Delivery of Mir-145, Carbohydrate Polymers (2017)
12. Development of 153Sm-Folate-Polyethyleneimine-Conjugated Chitosan Nanoparticles for Targeted Therapy, Journal of Labelled Compounds and Radiopharmaceuticals (2015)
13. Thiolated Carboxymethyl Dextran As a Nanocarrier for Colon Delivery of Hset1 Antisense: In Vitro Stability and Efficiency Study, Materials Science and Engineering C (2016)
14. Introducing Electrospray As a Potent Technique to Deliver Chitosan/Pdna Nanoparticles to Eukaryotic Cells, Journal of Nano Research (2021)
15. Stimuli-Sensitive Chitosan-Based Nanosystems-Immobilized Nucleic Acids for Gene Therapy in Breast Cancer and Hepatocellular Carcinoma, Current Topics in Medicinal Chemistry (2024)
16. Modeling and Optimization of Degree of Folate Grafted on Chitosan and Carboxymethyl-Chitosan, Progress in Biomaterials (2016)
17. Application of Chitosan in Oral Drug Delivery, Functional Chitosan: Drug Delivery and Biomedical Applications (2020)
18. Inhibiting Influenza Virus Replication and Inducing Protection Against Lethal Influenza Virus Challenge Through Chitosan Nanoparticles Loaded by Sirna, Drug Delivery and Translational Research (2018)
19. Nanoparticles Prepared From N,N-Dimethyl-N-Octyl Chitosan As the Novel Approach for Oral Delivery of Insulin: Preparation, Statistical Optimization and In-Vitro Characterization, Iranian Journal of Pharmaceutical Research (2018)
20. Development of Octreotide-Loaded Chitosan and Heparin Nanoparticles: Evaluation of Surface Modification Effect on Physicochemical Properties and Macrophage Uptake, Journal of Pharmaceutical Sciences (2019)