Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System

Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System Publisher

Rafiee A¹ ; Riazirad F² ; Alimohammadian MH² ; Gazori T³ ; Fatemi SMR⁴ ; Havaskary M⁵

Show Affiliations

1. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2. Immunology Department, Pasteur Institute of Iran, Tehran, Iran
3. Department of Pharmaceutics, Tehran University of Medical Sciences (TUMS), Tehran, Iran
4. Science and Research Branch, Department of Marine Biology, Islamic Azad University, Tehran, Iran
5. Young Researchers Club, Tehran Central Branch, Islamic Azad University, Tehran, Iran

Source: Cytology and Genetics Published:2015

Abstract

To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles. © 2015, Allerton Press, Inc.

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Style	Citing Format
MLA	Rafiee A, et al.. "Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System." Cytology and Genetics, vol. 49, no. 2, 2015, pp. 90-94.
APA	Rafiee A, Riazirad F, Alimohammadian MH, Gazori T, Fatemi SMR, Havaskary M (2015). Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System. Cytology and Genetics, 49(2), 90-94.
Chicago	Rafiee A, Riazirad F, Alimohammadian MH, Gazori T, Fatemi SMR, Havaskary M. "Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System." Cytology and Genetics 49, no. 2 (2015): 90-94.
Harvard	Rafiee A et al. (2015) 'Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System', Cytology and Genetics, 49(2), pp. 90-94.
Vancouver	Rafiee A, Riazirad F, Alimohammadian MH, Gazori T, Fatemi SMR, Havaskary M. Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System. Cytology and Genetics. 2015;49(2):90-94.
BibTex	@article{ author = {Rafiee A and Riazirad F and Alimohammadian MH and Gazori T and Fatemi SMR and Havaskary M}, title = {Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System}, journal = {Cytology and Genetics}, volume = {49}, number = {2}, pages = {90-94}, year = {2015} }
RIS	TY - JOUR AU - Rafiee A AU - Riazirad F AU - Alimohammadian MH AU - Gazori T AU - Fatemi SMR AU - Havaskary M TI - Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System JO - Cytology and Genetics VL - 49 IS - 2 SP - 90 EP - 94 PY - 2015 ER -

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