Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System

Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System Publisher

Rafiee A¹ ; Riazirad F² ; Alimohammadian MH² ; Gazori T³ ; Fatemi SMR⁴ ; Havaskary M⁵

Source: Cytology and Genetics Published:2015

Abstract

To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles. © 2015, Allerton Press, Inc.

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Style	Citing Format
MLA	Rafiee A, et al.. "Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System." Cytology and Genetics, vol. 49, no. 2, 2015, pp. 90-94.
APA	Rafiee A, Riazirad F, Alimohammadian MH, Gazori T, Fatemi SMR, Havaskary M (2015). Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System. Cytology and Genetics, 49(2), 90-94.
Chicago	Rafiee A, Riazirad F, Alimohammadian MH, Gazori T, Fatemi SMR, Havaskary M. "Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System." Cytology and Genetics 49, no. 2 (2015): 90-94.
Harvard	Rafiee A et al. (2015) 'Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System', Cytology and Genetics, 49(2), pp. 90-94.
Vancouver	Rafiee A, Riazirad F, Alimohammadian MH, Gazori T, Fatemi SMR, Havaskary M. Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System. Cytology and Genetics. 2015;49(2):90-94.
BibTex	@article{ author = {Rafiee A and Riazirad F and Alimohammadian MH and Gazori T and Fatemi SMR and Havaskary M}, title = {Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System}, journal = {Cytology and Genetics}, volume = {49}, number = {2}, pages = {90-94}, year = {2015} }
RIS	TY - JOUR AU - Rafiee A AU - Riazirad F AU - Alimohammadian MH AU - Gazori T AU - Fatemi SMR AU - Havaskary M TI - Hydrogel Nanoparticle Encapsulated Plasmid As a Suitable Gene Delivery System JO - Cytology and Genetics VL - 49 IS - 2 SP - 90 EP - 94 PY - 2015 ER -

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