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Novel Chitosan Based Nanoparticles As Gene Delivery Systems to Cancerous and Noncancerous Cells Publisher Pubmed



Rahmani S1 ; Hakimi S2 ; Esmaeily A2 ; Samadi FY3 ; Mortazavian E4 ; Nazari M5 ; Mohammadi Z3, 6 ; Tehrani NR7 ; Tehrani MR8
Authors
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Authors Affiliations
  1. 1. Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences (TUMS), Tehran, Iran
  2. 2. Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  4. 4. Research and Development Department, Borna Pharmed Pharmaceutical Company, Tehran, Iran
  5. 5. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  6. 6. School of Pharmacy-International Campus, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Departments of Pharmaceutics and Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Pharmaceutics Published:2019


Abstract

The present study aimed to investigate in vitro DNA transfection efficiency of three novel chitosan derivatives: thiolated trimethyl chitosan (TMC-Cys), methylated 4-N,N dimethyl aminobenzyl N,O carboxymethyl chitosan(MABCC) and thiolated trimethyl aminobenzyl chitosan(MABC-Cys). After polymer synthesis and characterization, nanoparticles were prepared using these polymers and their size, zeta potential and DNA condensing ability were measured. After that, cytotoxicity and transfection efficiency of nanocomplexes were carried out in three different cells. The results showed that all polymers could condense DNA plasmid strongly from N/P 2 and nanocomplexes had eligible sizes and zeta potentials. Moreover, the nanocomplexes had negligible cytotoxicity and MABC-Cys was the most effective vehicle for gene delivery in HEK-293T cells. In the two other cell lines, SKOV-3 and MCF-7, TMC-Cys exhibited the highest transfection efficiency. This study indicated that chemical structure of these novel chitosan derivatives in the interaction with the cell type can lead to successful gene delivery. © 2019 Elsevier B.V.
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