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Humanized Culture Medium for Clinical-Grade Generation of Erythroid Cells From Umbilical Cord Blood Cd34+ Cells Publisher



Zamani M1 ; Yaghoubi Y2 ; Naimi A3 ; Hassanzadeh A4 ; Pourakbari R2 ; Aghebatimaleki L5 ; Motavalli R2 ; Aghlmandi A2 ; Mehdizadeh A6 ; Nazari M7 ; Yousefi M2 ; Movassaghpour AA5, 8
Authors
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Authors Affiliations
  1. 1. Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
  2. 2. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  7. 7. Department of Anesthesiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  8. 8. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Advanced Pharmaceutical Bulletin Published:2021


Abstract

Purpose: Transfusion of red blood cells (RBCs) is a supportive and common treatment in surgical care, trauma, and anemia. However, in vivo production of RBC seems to be a suitable alternative for blood transfusions due to the limitation of blood resources, the possibility of disease transmission, immune reactions, and the presence of rare blood groups. Cell cultures require serum-free or culture media supplemented with highly expensive animal serum, which can transmit xenoviruses. Platelet lysate (PL) can be considered as a suitable alternative containing a high level of growth factors and a low production cost. Methods: Three-step culture media supplemented with PL or fetal bovine serum (FBS) were used for proliferation and differentiation of CD34+ umbilical cord blood stem cells to erythrocytes in co-culture with bone marrow mesenchymal stem cells (BM-MSCs). The cells were cultivated for 15 days and cell proliferation and expansion were assessed using cell counts at different days. Erythroid differentiation genes, CD71 and glycophorin A expression levels were evaluated. Results: Maximum hematopoietic stem cells (HSCs) proliferation was observed on day 15 in PL-containing medium (99±17×103-fold). Gene expression and surface markers showed higher differentiation of cells in PL-containing medium. Conclusion: The results of this study indicate that PL can enhance erythroid proliferation and differentiation of CD34+ HSCs. PL can also be used as a proper alternative for FBS in the culture medium and HSCs differentiation. © 2021 The Author (s).
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