Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
P2 Receptors Mrna Expression Profiles in Macrophages From Ankylosing Spondylitis Patients and Healthy Individuals Publisher Pubmed



Akhtari M1, 2 ; Zargar SJ1 ; Vojdanian M2 ; Ashrafganjouei A2 ; Javinani A2 ; Hamzeh E2 ; Rezaiemanesh A3 ; Jamshidi A2, 4 ; Mahmoudi M2, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
  2. 2. Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
  4. 4. Inflammation Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Rheumatic Diseases Published:2020


Abstract

Background: Ankylosing spondylitis (AS) is a multifactorial rheumatic disease which mainly involves the axial skeleton. Macrophages and extracellular nucleotides have been shown to contribute to the inflammation process in autoimmune diseases. Membrane-bound purinergic P2 receptors might be involved in the modulation of immune cells in AS. Therefore, we aimed to analyze the messenger RNA (mRNA) expression of P2 receptors in the macrophages of AS patients and healthy controls. Methods: Twenty-three AS patients and 23 age- and sex-matched healthy individuals were included in our study. Whole blood-separated monocytes of study participants were stimulated by macrophage colony-stimulating factor for 7 days and differentiated to macrophages. Monocyte and macrophage markers were analyzed by flow cytometry. SYBR green real-time polymerase chain reaction was used to measure the relative expression levels of P2RX1, P2RX2, P2RX3, P2RX4, P2RX5, P2RX6, P2RX7, P2RY1, P2RY2, P2RY4, P2RY6, P2RY11, P2RY12, P2RY13, P2RY14, and PANX1 genes. Results: P2RY13 and P2RY6 genes had the highest expression levels in macrophages among P2RY genes. P2RY1 mRNA expression was significantly down-regulated (−1.75 fold) and P2RY14 was up-regulated (2.6 fold) in macrophages of AS patients compared to healthy individuals. P2RX4 gene had the highest expression in monocyte-derived macrophages, followed by P2RX7 and P2RX1genes. There was no significant difference in P2X receptor mRNA expression level between macrophages of AS patients and healthy individuals. Conclusions: Our results indicate that AS patients show altered expression levels of P2 receptor genes. Moreover, these changes might be associated with disease activity and patients’ status. © 2019 Asia Pacific League of Associations for Rheumatology and John Wiley & Sons Australia, Ltd
Related Docs
View other Related Docs
1. Downregulation of Itm2a Gene Expression in Macrophages of Patients With Ankylosing Spondylitis, International Archives of Allergy and Immunology (2021)
2. Monocyte-Derived and M1 Macrophages From Ankylosing Spondylitis Patients Released Higher Tnf-Α and Expressed More Il1b in Response to Bzatp Than Macrophages From Healthy Subjects, Scientific Reports (2021)
3. Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis, Clinical Immunology (2023)
4. Oral Administration Effects of Β-D-Mannuronic Acid (M2000) on Th17 and Regulatory T Cells in Patients With Ankylosing Spondylitis, Biomedicine and Pharmacotherapy (2018)
5. Association of the Genetic Variants in the Endoplasmic Reticulum Aminopeptidase 2 Gene With Ankylosing Spondylitis Susceptibility, International Journal of Rheumatic Diseases (2021)
6. Activation of Adenosine A2a Receptor Induced Interleukin-23 Mrna Expression in Macrophages of Ankylosing Spondylitis Patients, Cytokine (2020)
7. Arthritis and Inflammatory Conditions of the Hip, The Adult Hip: Hip Arthroplasty Surgery: Third Edition (2015)
8. Increased Inflammatory Responsiveness of Peripheral Blood Mononuclear Cells (Pbmcs) to in Vitro Nod2 Ligand Stimulation in Patients With Ankylosing Spondylitis, Immunopharmacology and Immunotoxicology (2018)
Experts (# of related papers)
View all Related Experts
Ahmad Reza Jamshidi (8)
Mahdi Mahmoudi (8)
Other Related Docs
9. A2a Adenosine Receptor Agonist Reduced Mmp8 Expression in Healthy M2-Like Macrophages But Not in Macrophages From Ankylosing Spondylitis Patients; [Authors and Affiliations], BMC Musculoskeletal Disorders (2022)
10. Single-Stage Bilateral Conversion Arthroplasty for Hip Fusion Via Direct Anterior Approach in a Patient With Severe Ankylosing Spondylitis and Kyphoscoliosis: A Case Report, Journal of Medical Case Reports (2023)
11. Ankylosing Spondylitis Monocyte-Derived Macrophages Express Increased Level of A2a Adenosine Receptor and Decreased Level of Ectonucleoside Triphosphate Diphosphohydrolase-1 (Cd39), A1 and A2b Adenosine Receptors, Clinical Rheumatology (2018)
12. Evaluation of the Efficacy and Safety of Β-D-Mannuronic Acid in Patients With Ankylosing Spondylitis: A 12-Week Randomized, Placebo-Controlled, Phase I/Ii Clinical Trial, International Immunopharmacology (2018)
13. Association Study Between Killer Immunoglobulin-Like Receptor Polymorphisms and Ankylosing Spondylitis Disease: An Updated Meta-Analysis, International Journal of Rheumatic Diseases (2018)