Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis

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Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis Publisher Pubmed

Babaie F^{1, 2} ; Mohammadi H^{3, 4} ; Salimi S^{5, 6} ; Ghanavatinegad A⁷ ; Abbasifard M^{8, 9} ; Yousefi M¹⁰ ; Hajaliloo M¹¹ ; Khalili Y¹² ; Zamanlou S¹² ; Safari R¹³ ; Hemmatzadeh M¹⁰ ; Rezaiemanesh A¹⁴ ; Salimi R¹⁵ ; Baradaran B^{5, 10} Show All Authors

Babaie F^{1, 2}
Mohammadi H^{3, 4}
Salimi S^{5, 6}
Ghanavatinegad A⁷
Abbasifard M^{8, 9}
Yousefi M¹⁰
Hajaliloo M¹¹
Khalili Y¹²
Zamanlou S¹²
Safari R¹³
Hemmatzadeh M¹⁰
Rezaiemanesh A¹⁴
Salimi R¹⁵
Baradaran B^{5, 10}
Babaloo Z^{5, 10, 11}

Show Affiliations

1. Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran
2. Department of Genetic and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
3. Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran
4. Department of Immunology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
5. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
7. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
8. Immunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
9. Department of Internal Medicine, Ali-Ibn Abi-Talib Hospital, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
10. Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
11. Connective Tissue Research Center, Tabriz University of Medical Science, Iran
12. Iranian Social Security Organization, Emam Reza Hospital, Urmia, Iran
13. Department of Infectious Disease, Imperial College London, London, United Kingdom
14. Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
15. Research and Development (R&D) Department, &RS Scientific, RS, Belgium

Source: Clinical Immunology Published:2023

Abstract

Background: We aimed to assess if Endoplasmic reticulum aminopeptidase 1 (ERAP1) polymorphisms might impress Human leukocyte antigen (HLA)-B27-free heavy chains (FHCs) expression on macrophages and eventually NK cell activation in Ankylosing spondylitis (AS). Methods: Blood samples were obtained from 10 HLA[sbnd]B27+ patients with protective and 10 HLA[sbnd]B27+ patients with non-protective genotype. Monocytes were isolated and polarized toward M1 and M2 macrophages. ERAP1 was inhibited in macrophages, which were then co-cultured with autologous NK cells. Results: Expression of HLA-B27-FHCs on M1 and M2 macrophages was reduced in patients with protective ERAP1 genotype. Co-culturing ERAP1-inhibited M1 macrophages and NK cells from patients with protective genotype resulted in downmodulation of CD69 and CD107a markers on NK cells and reduced number of IFN-γ+ NK cells compared to that of patients with non-protective genotypes. Conclusion: Inhibition of ERAP1 activity, by diminishing NK activation, may have therapeutic value in treating AS patients. © 2023 Elsevier Inc.

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Style	Citing Format
MLA	Babaie F, et al.. "Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis." Clinical Immunology, vol. 248, no. , 2023, pp. -.
APA	Babaie F, Mohammadi H, Salimi S, Ghanavatinegad A, Abbasifard M, Yousefi M, Hajaliloo M, Khalili Y, Zamanlou S, Safari R, Hemmatzadeh M, Rezaiemanesh A, Salimi R, Baradaran B, Babaloo Z (2023). Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis. Clinical Immunology, 248(), -.
Chicago	Babaie F, Mohammadi H, Salimi S, Ghanavatinegad A, Abbasifard M, Yousefi M, Hajaliloo M, et al.. "Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis." Clinical Immunology 248, no. (2023): -.
Harvard	Babaie F et al. (2023) 'Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis', Clinical Immunology, 248(), pp. -.
Vancouver	Babaie F, Mohammadi H, Salimi S, Ghanavatinegad A, Abbasifard M, Yousefi M, et al.. Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis. Clinical Immunology. 2023;248():-.
BibTex	@article{ author = {Babaie F and Mohammadi H and Salimi S and Ghanavatinegad A and Abbasifard M and Yousefi M and Hajaliloo M and Khalili Y and Zamanlou S and Safari R and Hemmatzadeh M and Rezaiemanesh A and Salimi R and Baradaran B and Babaloo Z}, title = {Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis}, journal = {Clinical Immunology}, volume = {248}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Babaie F AU - Mohammadi H AU - Salimi S AU - Ghanavatinegad A AU - Abbasifard M AU - Yousefi M AU - Hajaliloo M AU - Khalili Y AU - Zamanlou S AU - Safari R AU - Hemmatzadeh M AU - Rezaiemanesh A AU - Salimi R AU - Baradaran B AU - Babaloo Z TI - Inhibition of Erap1 Represses Hla-B27 Free Heavy Chains Expression on Polarized Macrophages and Interrupts Nk Cells Activation and Function From Ankylosing Spondylitis JO - Clinical Immunology VL - 248 IS - SP - EP - PY - 2023 ER -

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