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Comparison of the Toxoplasma Gondii Mice and Cell Culture Derived Antigens in Elisa Assay Pubmed



Poshtehban A1 ; Shojaee S1 ; Keshavarz H1 ; Salimi M1 ; Mohebali M1
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Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Tropical Biomedicine Published:2017


Abstract

Toxoplasmosis is an infectious disease caused by the coccidian parasite Toxoplasma gondii. Diagnosis is based on serological methods with detection of specific IgG and IgM antibodies. The present study was performed to compare the sensitivity and specificity of soluble antigen of T. gondii, RH strain obtained from mice and cell culture in ELISA method. Tachyzoites of T. gondii, RH strain that inoculated in mice peritoneum were collected. At the same time, tachyzoites were harvested from HeLa cell culture that infected with the parasite. Soluble antigen was prepared and ELISA method performed on 100 serum samples that were collected from different laboratories in Tehran, Iran. Commercial Trinity kit was used as gold standard. The sensitivity and specificity of T.gondii soluble antigen were higher in antigens that obtained from cell culture in comparison with mice peritoneum. T. gondii cell culture derived antigen has high sensitivity and specificity in ELISA test. © 2017, Malaysian Society for Parasitology. All rights reserved.
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Hossein Keshavarz Valian (9)
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