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Fabrication of Bioartificial Pancreas Using Decellularized Rat Testicular Tissue Publisher Pubmed



Karimi S1 ; Khorsandi LS2 ; Ai J3
Authors
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Authors Affiliations
  1. 1. Abadan Faculty of Medical Science, Abadan, Iran
  2. 2. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  3. 3. Tissue Engineering and Applied Cell Sciences, Department-School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta Histochemica Published:2022


Abstract

Aims: Diabetes is a chronic disease that is associated with a decrease or disfunction of β-cell. In the present study, fabrication of bioartificial pancreas using MIN-6 β-cell line seeded in decellularized rat testicles was investigated. Main methods: In this experimental study, the whole body of testes were decellularized and after characterization, were seeded by MIN-6 cell line. The expression of insulin-related genes and proteins including PDX-1, Glut2, Insulin, and Neurogenin-3 were evaluated. Insulin secretion was assessed under different concentrations of glucose. Seeded scaffolds with or without MIN-6 cells were transplanted to the rat's mesentery and their blood sugar and body weight were evaluated every three days for 28 days and analyzed with H&E staining. Results: Histological assessments indicated the cells were completely removed after decellularization. The scaffold had no toxic impacts on the MIN-6 cells (P˂ 0.02). Insulin release in response to different concentrations of glucose in 3D culture (testis-ECM) was significantly more than the traditional 2D monolayer culture (P < 0.001). Moreover, the relative genes and proteins expression were significantly higher in the 3D culture, compared to the 2D control group. In vivo transplantation of the (testis- Extra Cellular Matrix) testis-ECM scaffolds showed appropriate positions for transplantation with angiogenesis and low infiltration of inflammatory cells. The recellularized scaffolds could drop blood sugar levels and increase the body-weight of STZ-diabetic rats (P < 0.01). Significance: Our study clearly confirmed that ECM valuable organ scaffolds prepared by decellularization of the testicular tissue is suitable for the fabrication of bioartificial pancreas for transplantation. © 2022 Elsevier GmbH