Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Cholestasis and Neuroaid Treatment on the Expression of Bax, Bcl-2, Pgc-1Α and Tfam Genes Involved in Apoptosis and Mitochondrial Biogenesis in the Striatum of Male Rats Publisher Pubmed



Nasehi M1 ; Torabinejad S2 ; Hashemi M2 ; Vaseghi S1 ; Zarrindast MR3, 4, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University, P.O. Box 13145-784, Tehran, Iran
  2. 2. Department of Genetics, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  3. 3. Department of Pharmacology School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Institute for Cognitive Science Studies (ICSS), Tehran, Iran
  5. 5. Department of Neuroendocrinology, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Metabolic Brain Disease Published:2020


Abstract

Cholestasis means impaired bile synthesis or secretion. In fact, it is a bile flow reduction following Bile Duct Ligation (BDL). Cholestasis has a main role in necrosis and apoptosis. Apoptosis is a form of programmed cell death that has intrinsic and extrinsic pathways. The intrinsic pathway is mediated by Bcl-2 (B cell lymphoma-2) proteins which integrate death and survival signals. Bcl-2 has anti-apoptotic and Bax has pro-apoptotic effects. Also, striatum is one of the brain regions that has high expressions of Bcl-2 proteins. Moreover, Tfam and Pgc-1α are involved in mitochondrial biogenesis. On the other hand, NeuroAid, is a drug that has neuroprotective and anti-apoptosis effects. In this study, using quantitative PCR, we measured the expression of all these genes in the striatum of male rats following BDL and NeuroAid administration. Results showed, BDL increased the expression of Bax and Tfam and decreased the expression of Bcl-2. NeuroAid restored the effect of BDL on the expression of Bax, while did not alter the effect of BDL on Bcl-2. In addition, it increased the expression of Tfam that was previously elevated by BDL and raised the expression of Tfam in normal rats. Both BDL and NeuroAid, had no effect on Pgc-1α. In conclusion, cholestasis increased the expression of Bax and decreased the expression of Bcl-2, and this effect may have related to enhanced susceptibility of mitochondrial pathways following oxidative stress. Tfam expression was increased following cholestasis and this effect may have related to cellular compensatory mechanisms against high accumulation of free radicals or mitochondrial biogenesis failure. Furthermore, NeuroAid may play a role against apoptosis and can be used to increase mitochondrial biogenesis. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
Related Docs
View other Related Docs
1. The Neuroprotective Effect of Neuroaid on Morphine-Induced Amnesia With Respect to the Expression of Tfam, Pgc-1Α, Δfosb and Cart Genes in the Hippocampus of Male Wistar Rats, Gene (2020)
2. Cholestasis and Behavioral Disorders, Gastroenterology and Hepatology from Bed to Bench (2021)
3. Antidepressant Efficacy of Mlc901 in the 6-Hydroxydopamine Mice Model of Parkinson’S Disease, Physiology and Pharmacology (Iran) (2021)
4. Evaluation of Prepulse Inhibition and Memory Impairments at Early Stage of Cirrhosis May Be Considered As a Diagnostic Index for Minimal Hepatic Encephalopathy, Physiology and Behavior (2017)
5. The Effects of Intra-Dorsal Hippocampus Infusion of Pregnenolone Sulfate on Memory Function and Hippocampal Bdnf Mrna Expression of Biliary Cirrhosis-Induced Memory Impairment in Rats, Neuroscience (2015)
6. Aromatic Hydrocarbon Receptors in Mitochondrial Biogenesis and Function, Mitochondrion (2021)
7. Possible Involvement of the Opioidergic System in the Modulation of Body Temperature, Jumping Behavior and Memory Process in Cholestatic and Addicted Mice, EXCLI Journal (2020)
8. Melatonin Application in Targeting Oxidative-Induced Liver Injuries: A Review, Journal of Cellular Physiology (2018)
Experts (# of related papers)
View all Related Experts
Ahmad Reza Dehpour (2)
Mohammad Ali Mansournia (2)
Other Related Docs
9. Cell Death Pathways and Viruses: Role of Micrornas, Molecular Therapy Nucleic Acids (2021)
10. Does the Use of Melatonin Overcome Drug Resistance in Cancer Chemotherapy?, Life Sciences (2018)
11. A Possible Neuroprotective Property of Ethanol And/Or Neuroaid on the Modulation of Cognitive Function, Neurotoxicology and Teratology (2020)
12. Curcumin Anti‐Tumor Effects on Endometrial Cancer With Focus on Its Molecular Targets, Cancer Cell International (2021)
13. Non-Coding Rnas and Brain Tumors: Insights Into Their Roles in Apoptosis, Frontiers in Cell and Developmental Biology (2022)
14. Development of a Bioluminescence Assay for Bir2- Caspase3 Interaction Through Split Luciferase Complementary Assay, Biochemical Engineering Journal (2022)
15. Resveratrol and the Mitochondria: From Triggering the Intrinsic Apoptotic Pathway to Inducing Mitochondrial Biogenesis, a Mechanistic View, Biochimica et Biophysica Acta - General Subjects (2016)
16. The Potential of the Incorporated Collagen Microspheres in Alginate Hydrogel As an Engineered Three-Dimensional Microenvironment to Attenuate Apoptosis in Human Pancreatic Islets, Acta Histochemica (2021)
17. Variants in the Ppargc1a Gene May Influence the Effect of Fat Intake on Resting Metabolic Rate in Obese Women, Lipids (2018)
18. Beta-Glucans Is a Potential Inhibitor of Ovarian Cancer: Based on Molecular and Biological Aspects, Current Pharmaceutical Biotechnology (2022)
19. The Role of Peroxisome Proliferator-Activated Receptor-Coactivator-1 Gene in Skin Aging, Journal of Research in Medical Sciences (2016)
20. A Comprehensive Insight Into Apoptosis: Molecular Mechanisms, Signaling Pathways, and Modulating Therapeutics, Cancer Investigation (2025)
21. Mlc901 During Sleep Deprivation Rescues Fear Memory Disruption in Rats, Naunyn-Schmiedeberg's Archives of Pharmacology (2019)
22. Design and Synthesis of Novel Ureido and Thioureido Conjugated Hydrazone Derivatives With Potent Anticancer Activity, BMC Chemistry (2022)