Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effects of Intra-Dorsal Hippocampus Infusion of Pregnenolone Sulfate on Memory Function and Hippocampal Bdnf Mrna Expression of Biliary Cirrhosis-Induced Memory Impairment in Rats Publisher Pubmed



Dastgheib M1 ; Dehpour AR2 ; Heidari M3 ; Moezi L1, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Experimental research center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Neuroscience Published:2015


Abstract

Learning and memory impairment is one of the most challenging complications of cirrhosis and present treatments are unsatisfactory. The exact mechanism of cirrhosis cognitive dysfunction is unknown. Pregnenolone sulfate (PREGS) is an excitatory neurosteroid that acts as a N-methyl- d-aspartate (NMDA) receptor agonist and GABAA receptor antagonist. In this study we evaluated the effect of intra CA1 infusion of PREGS on cirrhotic rats' memory function using the Y-maze test. Hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression was also evaluated. Three weeks after bile duct ligation (BDL) surgery, rats were under stereotaxic surgery for insertion of two guide cannulas in the CA1 region of the hippocampus. After 1-week of recovery, PREGS was administered through CA1 cannulas in cirrhotic rats, while control or sham groups received vehicle. For evaluation of NMDA receptor role in memory-enhancing effects of PREGS, DL-2-Amino-5-phosphonopentanoic acid (AP5) which is a potent and competitive antagonist of NMDA receptor, co-administered with PREGS and for assessment of hippocampal BDNF mRNA expression, quantitative Real-time reverse transcriptase-PCR (RT-PCR) was used. Results showed that 28. days after BDL, cirrhotic animals' memory significantly decreased in comparison with control and sham groups, while PREGS infusion could restore memory impairment (P< 0.05). PREGS effects on memory of cirrhotic rats were antagonized by DAP5. RT-PCR findings have shown that hippocampal relative BDNF mRNA expression was up-regulated in PREGS-treated groups in comparison with the BDL group (P< 0.001). Our findings suggest that PREGS has a memory-enhancing effect in cirrhosis memory deficit in acute therapy and this effect may be through NMDA (glutamate) receptor involvement and BDNF mRNA expression. © 2015 IBRO.
Related Docs
View other Related Docs
1. The Modulatory Effect of Ca1 Gabab Receptors on Ketamine-Induced Spatial and Non-Spatial Novelty Detection Deficits With Respect to Ca2+, Neuroscience (2015)
2. Cholestasis and Behavioral Disorders, Gastroenterology and Hepatology from Bed to Bench (2021)
3. Activation of Trpv1 Receptors Affects Memory Function and Hippocampal Trpv1 and Creb Mrna Expression in a Rat Model of Biliary Cirrhosis, Neurological Research (2018)
4. Function of Opioidergic and Dopaminergic Antagonists on Both Spatial and Object Novelty Detection Deficits Induced in Rodent Model of Hepatic Encephalopathy, Behavioural Brain Research (2016)
5. Role of Ca1 Gabaa and Gabab Receptors on Learning Deficit Induced by D-Ap5 in Passive Avoidance Step-Through Task, Brain Research (2018)
6. Possible Involvement of the Opioidergic System in the Modulation of Body Temperature, Jumping Behavior and Memory Process in Cholestatic and Addicted Mice, EXCLI Journal (2020)
7. Synergistic Effect Between D-Ap5 and Muscimol in the Nucleus Accumbens Shell on Memory Consolidation Deficit in Adult Male Wistar Rats: An Isobologram Analysis, Neurobiology of Learning and Memory (2017)
8. Enhanced Anxiolytic and Analgesic Effectiveness or a Better Safety Profile of Morphine and Tramadol Combination in Cholestatic and Addicted Mice, NeuroReport (2022)
Experts (# of related papers)
View all Related Experts
Nasim Vousooghi (6)
Ghorbangol Ashabi (3)
Other Related Docs
9. Effect of Cholestasis and Neuroaid Treatment on the Expression of Bax, Bcl-2, Pgc-1Α and Tfam Genes Involved in Apoptosis and Mitochondrial Biogenesis in the Striatum of Male Rats, Metabolic Brain Disease (2020)
10. The Modulatory Role of Dopamine in Anxiety-Like Behavior, Archives of Iranian Medicine (2015)
11. The Role of Nmda Receptors of the Medial Septum and Dorsal Hippocampus on Memory Acquisition, Pharmacology Biochemistry and Behavior (2016)
12. Different Role of Ca1 5Ht3 Serotonin Receptors on Memory Acquisition Deficit Induced Total(Tsd) and Rem Sleep Deprivation(Rsd), Archives of Iranian Medicine (2017)
13. Evaluation of Prepulse Inhibition and Memory Impairments at Early Stage of Cirrhosis May Be Considered As a Diagnostic Index for Minimal Hepatic Encephalopathy, Physiology and Behavior (2017)
14. The Involvement of Medial Septum 5-Ht1 and 5-Ht2 Receptors on Acpa-Induced Memory Consolidation Deficit: Possible Role of Trpc3, Trpc6 and Trpv2, Journal of Psychopharmacology (2015)
15. The Bidirectional Effect of Prelimbic 5-Hydroxytryptamine Type-4 (5-Ht4) Receptors on Acpa-Mediated Aversive Memory Impairment in Adult Male Sprague-Dawley Rats, Iranian Journal of Basic Medical Sciences (2021)
16. Recovery From Ketamine-Induced Amnesia by Blockade of Gaba-A Receptor in the Medial Prefrontal Cortex of Mice, Neuroscience (2017)
17. Dorsal Hippocampal Nmda Receptors Mediate the Interactive Effects of Arachidonylcyclopropylamide and Mdma/Ecstasy on Memory Retrieval in Rats, Progress in Neuro-Psychopharmacology and Biological Psychiatry (2016)
18. The Modulatory Role of Nicotine on Cognitive and Non-Cognitive Functions, Brain Research (2019)
19. The Effect of Ca1 Α2 Adrenergic Receptors on Memory Retention Deficit Induced by Total Sleep Deprivation and the Reversal of Circadian Rhythm in a Rat Model, Neurobiology of Learning and Memory (2016)
20. Cart Peptide and Opioid Addiction: Expression Changes in Male Rat Brain, Neuroscience (2016)
21. Does Pregnenolone Adjunct to Risperidone Ameliorate Irritable Behavior in Adolescents With Autism Spectrum Disorder: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial?, Clinical Neuropharmacology (2020)
22. Possible Involvement of the Ca1 Gabaa Receptors Upon Acquisition and Expression of the Acpa-Induced Place Preference in Mice, Physiology and Behavior (2016)
23. Parental Morphine Exposure Affects Repetitive Grooming Actions and Marble Burying Behavior in the Offspring: Potential Relevance for Obsessive-Compulsive Like Behavior, European Journal of Pharmacology (2019)
24. Evaluation of the Cart Peptide Expression in Morphine Sensitization in Male Rats, European Journal of Pharmacology (2017)
25. Is the Nociception Mechanism Altered in Offspring of Morphine-Abstinent Rats?, Journal of Pain (2018)
26. Synergistic Effect Between Prelimbic 5-Ht3 and Cb1 Receptors on Memory Consolidation Deficit in Adult Male Sprague-Dawley Rats: An Isobologram Analysis, Neuroscience (2016)
27. Involvement of Medial Prefrontal Cortex Alpha-2 Adrenoceptors on Memory Acquisition Deficit Induced by Arachidonylcyclopropylamide, a Cannabinoid Cb1 Receptor Agonist, in Rats; Possible Involvement of Ca2+ Channels, Journal of Psychopharmacology (2016)
28. Adult Rat Morphine Exposure Changes Morphine Preference, Anxiety, and the Brain Expression of Dopamine Receptors in Male Offspring, International Journal of Developmental Neuroscience (2018)
29. Interaction Between Dorsal Hippocampal Nmda Receptors and Lithium on Spatial Learning Consolidation in Rats, Brain Research Bulletin (2016)
30. Additive Effect of Bla Gabaa Receptor Mechanism and (+)-Mk-801 on Memory Retention Deficit, an Isobologram Analysis, Pharmacology Biochemistry and Behavior (2016)
31. Nmda Receptors of Blood Lymphocytes Anticipate Cognitive Performance Variations in Healthy Volunteers, Physiology and Behavior (2019)
32. The Effects of Ca1 5Ht4 Receptors in Mk801-Induced Amnesia and Hyperlocomotion, Neuroscience Letters (2015)
33. 5-Ht1a Receptor Blockade Targeting the Basolateral Amygdala Improved Stress-Induced Impairment of Memory Consolidation and Retrieval in Rats, Neuroscience (2015)
34. Critical Role of Ca1 Muscarinic Receptors on Memory Acquisition Deficit Induced by Total (Tsd) and Rem Sleep Deprivation (Rsd), Progress in Neuro-Psychopharmacology and Biological Psychiatry (2017)
35. Curcumin Prevents Cognitive Deficits in the Bile Duct Ligated Rats, Psychopharmacology (2020)