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Vitamin D Receptor Gene Polymorphisms and Risk of Breast Cancer in Iranian Women Publisher



Kazemi SM1, 2 ; Esmaielibandboni A3 ; Veisi Malekshahi Z4 ; Shahbaz Sardood M4 ; Hashemi M1 ; Majidzadeh K5 ; Kadkhodazadeh M6 ; Esmaili R5 ; Negahdari B4
Authors
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Authors Affiliations
  1. 1. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
  2. 2. Toxicology and Chemotherapy Unit, German Cancer Research Center (DKFZ), Heidelberg, Germany
  3. 3. Department of Medical Genetics, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  4. 4. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Genetics Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
  6. 6. Department of Virology, Pasteur Institute of Iran, Tehran, Iran

Source: Annals of Medicine and Surgery Published:2022


Abstract

Objectives: Vitamin D deficiency is a driving force of common cancers like breast cancer. Vitamin D receptor (VDR) can play a tumor suppressor role by helping the precise function of vitamin D in cells such as modulation TGF-β signaling pathway. This study aimed to investigate the association of VDR gene variants and susceptibility to breast cancer in Iranian women. Methods: Genomic DNAs were isolated from blood samples of 161 women with breast cancer and 150 healthy women. After amplification of five positions of VDR gene, the prepared amplicons were digested with TaqI, ApaI, BsmI, Cdx2, and FokI restriction enzymes. Results: Subsequently, the digested products were electrophoresed on the 1.5% agarose gel. Odds ratios (ORs) for breast cancer were calculated for genotypes and estimated haplotypes. Binary logistic regression analysis showed FokI (rs2228570), BsmI (rs1544410), and ApaI (rs7975232) polymorphisms had the significant distribution in patients than to the normal group. Analysis of linkage disequilibrium for all pairs of SNPs showed that D′-value between SNP TaqI and SNP BsmI was significantly (p ≤ 0.05). We observed that four major haplotypes of ApaI, BsmI, FokI, Cdx2, and TaqI SNPs significantly were in high frequency than predicted frequency. Among these four haplotypes, CGTAT haplotype was in a higher significant association than others with breast cancer risk (p-value = 0.0001). Conclusion: Our results showed that FokI, BsmI, and ApaI of VDR polymorphisms associated with the risk of breast cancer in Iranian population. © 2021 The Authors
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