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Formation of Self-Assembled Triple-Layered Rotavirus-Like Particles (Tlrlps) by Constitutive Co-Expression of Vp2, Vp6, and Vp7 in Stably Transfected High-Five Insect Cell Lines Publisher Pubmed



Shoja Z1, 2 ; Tagliamonte M3 ; Jalilvand S2 ; Mollaeikandelous Y2 ; De Stradis A4 ; Tornesello ML3 ; Buonaguro FM3 ; Buonaguro L3
Authors
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Authors Affiliations
  1. 1. Virology Department, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Molecular Biology and Viral Oncology, National Cancer Institute Fondazione Pascale, Naples, Italy
  4. 4. National Resource Council, Institute of Plant Virology, Bari, Italy

Source: Journal of Medical Virology Published:2015


Abstract

In this study, stable high-five insect cell line constitutively expressing rotavirus (RV) VP2 was co-transfected with VP6 and VP7-recombinant plasmids. The presence of RV proteins in stably transfected high-five cells was verified by molecular and protein analyses. To yield self-assembled triple-layered RV-like particles (tlRLPs), a stable insect high-five cell line was generated to produce RV VP6 and VP7 besides VP2. Self-assembled tlRLPs were observed by transmission electron microscopy (TEM), and enzyme-linked immunosorbent assay (ELISA) was used to assess their antigenicity in vivo. The results suggest that the stable transfected high-five cells are able to generate tlRLPs with the efficient antigenicity. © 2014 Wiley Periodicals, Inc.
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