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Co-Administration of Rotavirus Nanospheres Vp6 and Nsp4 Proteins Enhanced the Anti-Nsp4 Humoral Responses in Immunized Mice Publisher Pubmed



Afchangi A1 ; Jalilvand S1 ; Arashkia A2 ; Latifi T1 ; Farahmand M1 ; Abolghasem Shirazi MM2 ; Mousavi Nasab SD3 ; Marashi SM1 ; Roohvand F2 ; Shoja Z2
Authors
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Authors Affiliations
  1. 1. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Virology, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran

Source: Microbial Pathogenesis Published:2022


Abstract

Inconveniences associated with the efficacy and safety of the World Health Organization (WHO) approved/prequalified live attenuated rotavirus (RV) vaccines, sounded for finding alternative non-replicating modals and proper RV antigens (Ags). Herein, we report the development of a RV candidate vaccine based on the combination of RV VP6 nanospheres (S) and NSP4112-175 proteins (VP6S + NSP4). Self-assembled VP6S protein was produced in insect cells. Analyses by western blotting and transmission electron microscopy (TEM) indicated expression of VP6 trimer structures with sizes of ≥140 kDa and presence of VP6S. Four group of mice were immunized (2-dose formulation) intra-peritoneally (IP) by either¨VP6S + NSP4¨ or each protein alone (VP6S or NSP4112-175) emulsified in aluminium hydroxide or control. Results indicated that VP6S + NSP4 formulation induced significant anti-VP6 IgG (P < 0.001) and IgA (P < 0.05) as well as anti-NSP4 IgG (P < 0.001) and enhancement of protective immunity. Analyses of anti-VP6S and anti-NSP4 IgG subclass (IgG1 and IgG2a) showed IgG1/IgG2a ≥6 and IgG1/IgG2a ≥3 ratios, respectively indicating Th2 polarization of immune responses. The combination of VP6S + NSP4 proteins emulsified in aluminum hydroxide adjuvant might present a dual universal, efficient and cost-effective candidate vaccine against RV infection. © 2022 Elsevier Ltd
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