Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries

Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries Publisher Pubmed

Aarstehfar A¹ ; Daneshnia F¹ ; Kord M² ; Roudbary M³ ; Zarrinfar H⁴ ; Fang W⁵ ; Hashemi SJ^{2, 6} ; Najafzadeh MJ⁷ ; Khodavaisy S^{2, 8} ; Pan W⁵ ; Liao W⁵ ; Badali H⁹ ; Rezaie S² ; Zomorodian K¹⁰ Show All Authors

Aarstehfar A¹
Daneshnia F¹
Kord M²
Roudbary M³
Zarrinfar H⁴
Fang W⁵
Hashemi SJ^{2, 6}
Najafzadeh MJ⁷
Khodavaisy S^{2, 8}
Pan W⁵
Liao W⁵
Badali H⁹
Rezaie S²
Zomorodian K¹⁰
Hagen F¹
Boekhout T^{1, 5, 11}

Show Affiliations

1. Yeast Biodiversity Department, Westerdijk Fungal Biodiversity Institute, Utrecht, Netherlands
2. Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
4. Allergy Esearch Center, Mashhad University of Medical Sciences, Mashhad, Iran
5. Shanghai Key Laboratory of Molecular Medical Mycology, Department of Dermatology, Shanghai Institute of Medical Mycology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China
6. Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran
7. Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
8. Zoonoses Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
9. Department of Medical Mycology, Invasive Fungi Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
10. Department of Medical Mycology and Parasitology, Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
11. Yeast Biodiversity Department, Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, Netherlands

Source: Frontiers in Cellular and Infection Microbiology Published:2019

Abstract

Occurrence of non-Candida albicans Candida (NCAC) species that are associated with elevated MIC values and therapeutic failures are increasing. As a result, timely and accurate means of identification to the species level is becoming an essential part of diagnostic practices in clinical settings. In this study, 301 clinically isolated yeast strains recovered fromvarious anatomical sites [Blood (n=145), other sites (n=156)] were used to assess the accuracy and practicality of API 20C AUX and 21-plex PCR compared to MALDI-TOF MS and large subunit rDNA (LSU rDNA). MALDI-TOF MS correctly identified 98.33%of yeast isolates, 100%of top five Candida species, 95.7%of rare yeast species, while 1.3%of isolates weremisidentified. API 20C AUX correctly identified 83.7%of yeast isolates, 97.2% of top five Candida species, 61.8% of rare yeast species, while 16.2% of yeast isolates were misidentified. The 21-plex PCR, accurately identified 87.3% of yeast isolates, 100%of top five Candida species, 72%of rare yeast species, but it misidentified 1.3% of rare yeast species while 9.9% of whole yeast isolates were not identified. The combination of rapidity of 21-plex PCR and comprehensiveness of API 20C AUX, led to correct identification of 92% of included yeast isolates. Due to expensiveness of MALDI-TOF MS and sequencing, this combination strategy could be the most accurate and inexpensive alternative identification strategy for developing countries. Moreover, by the advent and development of cost-effective, reliable, and rapid PCRmachines that cost 130 US dollars, 21-plex could be integrated in routine laboratories of developing and resource-limited countries to specifically identify 95% causative agents of yeast-related infections in human. Databases of MALDI-TOF MS, API 20C AUX, and the number of target species identified by 21-plex require further improvement to keep up with the diverse spectrum of yeast species. © 2019 Frontiers Media S.A. All Rights Reserved.

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Style	Citing Format
MLA	Aarstehfar A, et al.. "Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries." Frontiers in Cellular and Infection Microbiology, vol. 9, no. , 2019, pp. -.
APA	Aarstehfar A, Daneshnia F, Kord M, Roudbary M, Zarrinfar H, Fang W, Hashemi SJ, Najafzadeh MJ, Khodavaisy S, Pan W, Liao W, Badali H, Rezaie S, Zomorodian K, Hagen F, Boekhout T (2019). Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries. Frontiers in Cellular and Infection Microbiology, 9(), -.
Chicago	Aarstehfar A, Daneshnia F, Kord M, Roudbary M, Zarrinfar H, Fang W, Hashemi SJ, et al.. "Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries." Frontiers in Cellular and Infection Microbiology 9, no. (2019): -.
Harvard	Aarstehfar A et al. (2019) 'Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries', Frontiers in Cellular and Infection Microbiology, 9(), pp. -.
Vancouver	Aarstehfar A, Daneshnia F, Kord M, Roudbary M, Zarrinfar H, Fang W, et al.. Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries. Frontiers in Cellular and Infection Microbiology. 2019;9():-.
BibTex	@article{ author = {Aarstehfar A and Daneshnia F and Kord M and Roudbary M and Zarrinfar H and Fang W and Hashemi SJ and Najafzadeh MJ and Khodavaisy S and Pan W and Liao W and Badali H and Rezaie S and Zomorodian K and Hagen F and Boekhout T}, title = {Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries}, journal = {Frontiers in Cellular and Infection Microbiology}, volume = {9}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Aarstehfar A AU - Daneshnia F AU - Kord M AU - Roudbary M AU - Zarrinfar H AU - Fang W AU - Hashemi SJ AU - Najafzadeh MJ AU - Khodavaisy S AU - Pan W AU - Liao W AU - Badali H AU - Rezaie S AU - Zomorodian K AU - Hagen F AU - Boekhout T TI - Comparison of 21-Plex Pcr and Api 20C Aux, Maldi-Tof Ms, and Rdna Sequencing for a Wide Range of Clinically Isolated Yeast Species: Improved Identification by Combining 21-Plex Pcr and Api 20C Aux As an Alternative Strategy for Developing Countries JO - Frontiers in Cellular and Infection Microbiology VL - 9 IS - SP - EP - PY - 2019 ER -

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