Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Prevalence and Molecular Mechanisms of Colistin Resistance in Acinetobacter Baumannii Clinical Isolates in Tehran, Iran Publisher Pubmed



Khoshbayan A1 ; Shariati A1 ; Shahmoradi S1 ; Baseri Z2 ; Mozafari H1 ; Darbansarokhalil D1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pathology and Laboratory Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta Microbiologica et Immunologica Hungarica Published:2021


Abstract

Colistin is one of the last remaining active antibiotics against multidrug resistant Gram-negative bacteria. However, several recent studies reported colistin-resistant (ColR) Acinetobacter baumannii from different countries. In the current study, we investigated molecular mechanisms involved in colistin resistance in A. baumannii isolates from different clinical samples. A total of 110 clinical A. baumannii isolates were collected from two hospitals in Tehran. Minimum inhibitory concentrations (MICs) were determined by broth microdilution according to the Clinical and Laboratory Standards Institute. For the ColR isolates, mutation was detected in pmrA, pmrB, lpxA, lpxC, and lpxD genes using the polymerase chain reaction (PCR) and sequencing. Moreover, the relative expression of the pmrC gene was calculated using quantitative reverse transcription PCR. Three colistin resistant isolates were identified with MIC between 8 and 16 μg/mL and were resistant to all the tested antimicrobial agents. All the three isolates had a mutation in the pmrB, pmrA, lpxA, lpxD, and lpxC genes. Moreover, the overexpression of pmrC gene was observed in all isolates. Our results showed that the upregulation of the PmrAB two component system was the primary mechanism linked to colistin resistance among the studied colistin resistant A. baumannii isolates. © 2021 Akademiai Kiado, Budapest.
Related Docs
View other Related Docs
1. Molecular Mechanisms of Colistin Resistance Among Pandrug-Resistant Isolates of Acinetobacter Baumannii With High Case-Fatality Rate in Intensive Care Unit Patients, Microbial Drug Resistance (2018)
2. Acquisition of Tn6018-3′ Cs Regions Increases Colistin Mics Against Acinetobacter Baumannii Isolates Harboring New Variants of Abars, Folia Microbiologica (2017)
3. Mutation in Mgrb Is the Major Colistin Resistance Mechanism in Klebsiella Pneumoniae Clinical Isolates in Tehran, Iran, Acta Microbiologica et Immunologica Hungarica (2022)
4. Comparison of Susceptibility Testing Methods for Determining the Activity of Colistin Against Gram-Negative Bacilli of Clinical Origin, Journal of Medical Microbiology (2019)
5. Growth Rate and Biofilm Formation Ability of Clinical and Laboratory-Evolved Colistin-Resistant Strains of Acinetobacter Baumannii, Frontiers in Microbiology (2018)
6. Mgrb Alterations Mediate Colistin Resistance in Klebsiella Pneumoniae Isolates From Iran, Frontiers in Microbiology (2017)
7. Plasmid Borne Carbapenem-Hydrolyzing Class D Β-Lactamases (Chdls) and Adeabc Efflux Pump Conferring Carbapenem-Tigecycline Resistance Among Acinetobacter Baumannii Isolates Harboring Tnabars, Microbial Pathogenesis (2017)
8. Evaluation of Photodynamic Therapy Effect Along With Colistin on Pandrug-Resistant Acinetobacter Baumannii, Laser Therapy (2017)
Experts (# of related papers)
View all Related Experts
Abbas Bahador (17)
Mohammad Mehdi Feizabadi (10)
Other Related Docs
9. Meropenem/Colistin Versus Meropenem/Ampicillin-Sulbactam in the Treatment of Carbapenem-Resistant Pneumonia, Journal of Comparative Effectiveness Research (2018)
10. Comparison of Ompa Gene-Targeted Real-Time Pcr With the Conventional Culture Method for Detection of Acinetobacter Baumanii in Pneumonic Balb/C Mice, Iranian Biomedical Journal (2019)
11. Variable Spontaneous Mutation Rate in Clinical Strains of Multidrug-Resistant Acinetobacter Baumannii and Differentially Expressed Proteins in a Hypermutator Strain, Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis (2017)
12. Antimicrobial Activity of Photodynamic Therapy in Combination With Colistin Against a Pan-Drug Resistant Acinetobacter Baumannii Isolated From Burn Patient, Photodiagnosis and Photodynamic Therapy (2017)
13. High Prevalence of Oxa-Type Carbapenemases Among Acinetobacter Baumannii Strains in a Teaching Hospital of Tehran, Acta Microbiologica et Immunologica Hungarica (2017)
14. An Increasing Threat in an Iranian Referral Children's Hospital: Multidrug-Resistant Acinetobacter Baumannii, Infectious Disorders - Drug Targets (2018)
15. Evidence of Interruption of the Comm Gene in a Large Series of Clinical Isolates of Multidrug-Resistant Acinetobacter Baumannii, Journal of Molecular Microbiology and Biotechnology (2016)
16. Clonal Relatedness and Biofilm Formation of Oxa-23-Producing Carbapenem Resistant Acinetobacter Baumannii Isolates From Hospital Environment, Microbial Pathogenesis (2016)
17. Modulation of Virulence in Acinetobacter Baumannii Cells Surviving Photodynamic Treatment With Toluidine Blue, Photodiagnosis and Photodynamic Therapy (2016)
18. Role of Efflux Pumps in Reduced Susceptibility to Tigecycline in Acinetobacter Baumannii, New Microbes and New Infections (2019)
19. Trend of Extensively Drug-Resistant Acinetobacter Baumannii and the Remaining Therapeutic Options: A Multicenter Study in Tehran, Iran Over a 3-Year Period, Letters in Applied Microbiology (2016)
20. First Report of Oxa-143-Lactamase Producing Acinetobacter Baumannii in Qom, Iran, Iranian Journal of Basic Medical Sciences (2017)
21. Molecular Evaluation of Colistin-Resistant Gene Expression Changes in Acinetobacter Baumannii With Real-Time Polymerase Chain Reaction, Infection and Drug Resistance (2017)
22. Combinatorial Therapy of Chitosan Hydrogel-Based Zinc Oxide Nanocomposite Attenuates the Virulence of Streptococcus Mutans, BMC Microbiology (2021)
23. Screening of Mutator Phenotype in Clinical Strains of Acinetobacter Baumannii, Microbial Pathogenesis (2017)
24. Antimicrobial-Resistance Pattern of Shigella Species in Children: A Six-Year Study in an Iranian Referral Hospital, Annali di Igiene Medicina Preventiva e di Comunita (2019)
25. Prevalence of Esbl and Mbl Encoding Genes in Acinetobacter Baumannii Strains Isolated From Patients of Intensive Care Units (Icu), Saudi Journal of Biological Sciences (2015)
26. The Threat of Colistin Resistance Among Carbapenem-Resistant Klebsiella Pneumoniae Isolates in Iran, Iranian Journal of Microbiology (2018)
27. Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella Spp. Recovered From Children With Diarrhea in Tehran, Iran, Infectious Disorders - Drug Targets (2020)
28. Evaluation of Acinetobacter Baumannii, Klebsiella Pneumoniae, and Staphylococcus Aureus Respiratory Tract Superinfections Among Patients With Covid-19 at a Tertiary-Care Hospital in Tehran, Iran, European Journal of Medical Research (2023)
29. Antimicrobial Resistance Patterns of Gram-Negative Bacteria Isolated From Bloodstream Infections in an Iranian Referral Paediatric Hospital: A 5.5-Year Study, Journal of Global Antimicrobial Resistance (2017)
30. Frequency of 16S Rrna Methylase and Aminoglycoside-Modifying Enzyme Genes Among Clinical Isolates of Acinetobacter Baumannii in Iran, Iranian Journal of Pathology (2017)
31. Prevalence of the Integrons and Esbl Genes in Multidrug-Resistant Strains of Escherichia Coli Isolated From Urinary Tract Infections, Ardabil, Iran, Iranian Journal of Medical Microbiology (2022)
32. The Β-Lactamase Disk Test: A Modified Method to Detect Extended-Spectrum-Β-Lactamases in Multidrug-Resistant Escherichia Coli Isolates, Archives of Clinical Infectious Diseases (2017)
33. Wide Distribution of Carbapenem Resistant Acinetobacter Baumannii in Burns Patients in Iran, Frontiers in Microbiology (2015)
34. Comparative In Vitro Activity of Carbapenems Against Clinical Isolates of Acinetobacter Baumannii, Journal of Applied Microbiology (2016)
35. In Vitro Antibiotic Combinations of Colistin, Meropenem, Amikacin, and Amoxicillin/Clavulanate Against Multidrug-Resistant Klebsiella Pneumonia Isolated From Patients With Ventilator-Associated Pneumonia, BMC Microbiology (2023)
36. The Prevalence of S. Aureus Skin and Soft Tissue Infections in Patients With Pemphigus, Autoimmune Diseases (2016)
37. Antimicrobial Resistance and Molecular Epidemiology of Virulence Genes Among Multi-Drug Resistant Acinetobacter Baumannii Clinical Isolates in Iran, Gene Reports (2021)
38. The Combination of Antimicrobial Photocatalysis and Antimicrobial Photodynamic Therapy to Eradicate the Extensively Drug-Resistant Colistin Resistant Acinetobacter Baumannii, Photodiagnosis and Photodynamic Therapy (2020)
39. The Impact of Primer Sets on Detection of the Gene Encoding Biofilm-Associated Protein (Bap) in Acinetobacter Baumannii: In Silico and In Vitro Analysis, Letters in Applied Microbiology (2017)
40. Characterization of Biofilm Formation, Antimicrobial Resistance, and Staphylococcal Cassette Chromosome Mec Analysis of Methicillin Resistant Staphylococcus Hominis From Blood Cultures of Children, Revista da Sociedade Brasileira de Medicina Tropical (2017)
41. Antimicrobial Resistance Profiles and Genetic Elements Involved in Carbapenem Resistance in Acinetobacter Baumannii Isolates From a Referral Hospital in Southern Iran, Journal of Global Antimicrobial Resistance (2016)
42. Molecular Characteristics of Staphylococcus Aureus Strains Isolated From Nasal Cavity and Wound Infections Among Diabetic Patients, Current Microbiology (2023)
43. Clinical Response and Outcome of Pneumonia Due to Multi-Drug Resistant Acinetobacter Baumannii in Critically Ill Patients, Iranian Journal of Microbiology (2016)
44. Diversity of Aminoglycoside Modifying Enzymes and 16S Rrna Methylases in Acinetobacter Baumannii and Acinetobacter Nosocomialis Species in Iran; Wide Distribution of Aada1 and Arma, Infection# Genetics and Evolution (2018)
45. Sub-Minimum Inhibitory Concentrations (Sub-Mics) of Colistin on Acinetobacter Baumannii Biofilm Formation Potency, Adherence, and Invasion to Epithelial Host Cells: An Experimental Study in an Iranian Children’S Referral Hospital, Microbiology Spectrum (2024)
46. Clonal Evolution Multi-Drug Resistant Acinetobacter Baumannii by Pulsed-Fi Eld Gel Electrophoresis, Indian Journal of Medical Microbiology (2015)
47. Molecular Analysis and Antimicrobial Resistance Pattern of Distinct Strains of Pseudomonas Aeruginosa Isolated From Cystic Fibrosis Patients in Iran, Iranian Journal of Microbiology (2019)
48. Mobile Genetic Elements Carrying Aminoglycoside Resistance Genes in Acinetobacter Baumannii Isolates Belonging to Global Clone 2, Frontiers in Microbiology (2023)
49. The Prevalence of Isaba1 and Isaba4 in Acinetobacter Baumannii Species of Different International Clone Lineages Among Patients With Burning in Tehran, Iran, Jundishapur Journal of Microbiology (2015)
50. Lytic Potential of a Filamentous Bacteriophage Isolated From Sewage Water in Tehran on Clinical Carbapenem-Resistant Strains, Iranian Journal of Microbiology (2022)