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Antioxidant and Antibacterial Activity of Safflower (Carthamus Tinctorius L.) Extract From Four Different Cultivars Publisher



Karimkhani MM1 ; Shaddel R2 ; Khodaparast MHH3 ; Vazirian M4 ; Pirigheshlaghi S5
Authors
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Authors Affiliations
  1. 1. School of Medicine, Gonabad University of Medical Sciences, Gonabad, 9691983643, Iran
  2. 2. Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, 5166614766, Iran
  3. 3. Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, 917751163, Iran
  4. 4. Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
  5. 5. Faculty of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, 4913815739, Iran

Source: Quality Assurance and Safety of Crops and Foods Published:2016


Abstract

Antioxidant and antimicrobial activities were studied in safflower methanolic extracts of four different cultivars (IL111, Padide, Isfahan-28 and Mahali). The total phenolic and flavonoid content of the plant extracts ranged from 46.2 to 62.3 mg gallic acid equivalent/g dry extract and 7.5 to 9.6 mg catechin equivalent/g dry extract, respectively. The antioxidant capacity of methanolic extracts was assessed by reducing power assay, DPPH• (2,2-diphenyl-1- picrylhydrazyl) scavenging activity and β-carotene bleaching methods. Results showed that cultivar 'IL111' had the highest phenolic and flavonoid content, as well as an antioxidant activity comparable to synthetic antioxidants. In addition, the antioxidant activity of the safflower extracts was evaluated in crude soybean oil by monitoring peroxide and thiobarbituric acid values, which showed a stabilisation effect on the soybean oil in the accelerated condition at 60 °C (oven test). Moreover, the analysis of phenolic compounds by HPLC showed that gallic acid was the predominant phenolic in safflower cultivar 'IL111'. The Isfahan-28 cultivar showed the best antimicrobial activity; the minimum inhibitory concentration against Staphylococcus aureus and Salmonella enterica serovar Typhi were 30 and 60 mg/ml, respectively. © 2016 Wageningen Academic Publishers.