Monitoring of Antifungal Drugs in Biological Samples Using Ultrasonic-Assisted Supramolecular Dispersive Liquid-Liquid Microextraction Based on Solidification of a Floating Organic Droplet Publisher Pubmed



Ezoddin M¹ ; Abdi K² Show Affiliations
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences Published:2016

Abstract

A new method for the simultaneous determination of the three antifungal drugs using ultrasonic-assisted supramolecular dispersive liquid-liquid microextraction based on solidification of a floating organic droplet (UASMDLLME-SFO) was proposed. The supramolecular solvents produced from reversed micelles of 1-dodecanol (extraction solvent) in tetrahydrofuran (THF) were injected into the aqueous sample solution. Reverse micelle coacervates were produced in situ through self-assembly processes. The antifungal drugs were extracted from the aqueous sample into a supramolecular solvent. Sonication accelerated the mass transfer of the target analytes into the supramolecular solvent phase and enhanced the dispersion process. Some parameters affecting the extraction efficiency such as type and volume of the extraction solvent, pH, volume of the disperser solvent and ultrasound extraction time were investigated. Under optimum conditions, the limits of detections for ketoconazole, clotrimazole and miconazole ranged from 0.08 to 1.3 μgL-1 and the relative standard deviations (RSDs, n = 5) < 6% were obtained. The method was successfully applied for preconcentration of the three drugs in biological and water samples. © 2016 Elsevier B.V.