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The Effect of Il-22 and Il-28 in Induction of Type 1 Regulatory T (Tr1) Cells Pubmed



Arasteh J1 ; Ebtekar M2 ; Pourpak Z3 ; Pourfatollah AA2 ; Hassan ZM2 ; Kardar GA3 ; Zare A3 ; Saghafi S3 ; Hassan ATM4
Authors
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Authors Affiliations
  1. 1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University Central Tehran Branch, Tehran, Iran
  2. 2. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Immunology Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, Faculty of Medical Sciences, Islamic Azad University Babol Branch, Babol, Iran

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2015


Abstract

Cytokines have been introduced as critical inducers in the development of Th subpopulations.Cytokines like IL-10 are involved in inducing regulatory T cells such as Type 1 regulatory T (Tr1) cells cells. IL-22 is a member of IL-10 family of cytokines, and IL-28A is a member of IFN-γ family. In this study, cord blood mononuclear cells (CBMC) from normal healthy individuals were isolated by Ficoll and then naive T cells were purified by CD4+CD25+ Regulatory T cell Isolation kit. The effect of these two cytokines on production of IL-5, TGF-β, IL-10, IL-4 and IFN-γ cytokines from cord blood T cells was investigated to identify Tr1 cells as well as Th1 and Th2 polarization. Flow cytometric analysis showed that IL-28A and IL-22 were not effective in expression of IL-5 and TGF-β either alone or in synergy, but in view of IL-10, IL-4 and IFN-γ, the results showed that IL-22 increased IL-10 and IL-4 but had a decreasing effect on IFN-γ. The results showed that IL-28A was not effective in increasing or decreasing the level of IL-10, IL-4 and IFN-γ. Therefore, according to these results, IL-22 and IL-28A were not effective in inducing Tr1 cells. Copyright © Spring 2015, Iran J Allergy Asthma Immunol. All rights reserved.
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