Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center

Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center Publisher

Mamishi S^{1, 2} ; Mohammadian M¹ ; Pourakbari B² ; Sadeghi RH² ; Ashtiani MTH³ ; Abdosalehi MR¹ ; Rahmani M⁴ ; Mahmoudi S²

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1. Department of Infectious Diseases, Pediatrics Center of Excellence, Childrenâ€™s Medical Center, Tehran, University of Medical Sciences, Tehran, Iran
2. Pediatric Infectious Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pathology, Pediatrics Center of Excellence, Childrenâ€™s Medical Center, Tehran University of Medical Sciences, Tehran, Iran
4. Pediatrics Center of Excellence, Childrenâ€™s Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Infection and Drug Resistance Published:2019

Abstract

Introduction: A serious problem affecting human society is the development of bacterial resistance. The purpose of the current study was to evaluate the antibiotic resistance of Grampositive bacteria (GPB) and genotyping of common GPB causing hospital-acquired infections (HAIs) in patients who were referred to Children’s Medical Center during a 6-month period by random amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus sequence polymerase chain reaction (ERIC-PCR). Methods: During the 6-month period, antimicrobial resistance profiles of GPB isolates recovered from patients in Children’s Medical Center were determined using the Kirby- Bauer disk diffusion and MIC. Typing of common GPB was performed and the results were analyzed by gel compare software. Results: In this cross-sectional study, 6524 cultures were performed and 138 Ggram-positive bacteria were isolated (2%). Staphylococcus aureus strains showed the highest antibiotic penicillin resistance (96.3%). Twenty-six per cent of the strains were methicillin-resistant S. aureus (MRSA) and no resistance was found against vancomycin. All isolates of Enterococcus faecium were resistant to ciprofloxacin (100%). The resistance to vancomycin was very high (67%) and no resistance was observed to linezolid. The results of genotyping analysis of S. epidermidis strains showed the presence of two clones with a genetic relationship of over 80%. All of the S. aureus strains were in one cluster and half of the E. faecium strains were in a cluster with a genetic predilection of over 80%. Conclusion: This study indicated frequent occurrence of antimicrobial resistance, especially in Enterococcus spp. isolates. Rapid spreads of MRSA and VREF from a clonal origin require implementing careful isolation and infection control measures. © 2019 Mamishi et al.

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Experts (# of related papers)

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Shima Mahmoudi (12)

Babak Pourakbari (12)

Style	Citing Format
MLA	Mamishi S, et al.. "Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center." Infection and Drug Resistance, vol. 12, no. , 2019, pp. 3719-3726.
APA	Mamishi S, Mohammadian M, Pourakbari B, Sadeghi RH, Ashtiani MTH, Abdosalehi MR, Rahmani M, Mahmoudi S (2019). Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center. Infection and Drug Resistance, 12(), 3719-3726.
Chicago	Mamishi S, Mohammadian M, Pourakbari B, Sadeghi RH, Ashtiani MTH, Abdosalehi MR, Rahmani M, Mahmoudi S. "Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center." Infection and Drug Resistance 12, no. (2019): 3719-3726.
Harvard	Mamishi S et al. (2019) 'Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center', Infection and Drug Resistance, 12(), pp. 3719-3726.
Vancouver	Mamishi S, Mohammadian M, Pourakbari B, Sadeghi RH, Ashtiani MTH, Abdosalehi MR, et al.. Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center. Infection and Drug Resistance. 2019;12():3719-3726.
BibTex	@article{ author = {Mamishi S and Mohammadian M and Pourakbari B and Sadeghi RH and Ashtiani MTH and Abdosalehi MR and Rahmani M and Mahmoudi S}, title = {Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center}, journal = {Infection and Drug Resistance}, volume = {12}, number = {}, pages = {3719-3726}, year = {2019} }
RIS	TY - JOUR AU - Mamishi S AU - Mohammadian M AU - Pourakbari B AU - Sadeghi RH AU - Ashtiani MTH AU - Abdosalehi MR AU - Rahmani M AU - Mahmoudi S TI - Antibiotic Resistance and Genotyping of Gram-Positive Bacteria Causing Hospital-Acquired Infection in Patients Referring to Children’S Medical Center JO - Infection and Drug Resistance VL - 12 IS - SP - 3719 EP - 3726 PY - 2019 ER -

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