Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa

Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa Publisher

Mardani A¹ ; Ziaei Hezarjaribi H² ; Fakhar M² ; Emadi SN^{3, 6} ; Rezaeirad A⁴ ; Butore J⁵ ; Claudete N⁵

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1. Department of Microbiology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2. Molecular and Cell Biology Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3. Skin Research Center, Razi Hospital, Tehran University of Medical Sciences (TUMS), Iran
4. Student Research Committee, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5. University Teaching Hospital of Kamenge (UTHK), Bujumbura, Burundi
6. Skin Research Center, Razi Hospital, Tehran University of Medical Sciences (TUMS) Tehran, P.O Box: 484751-91971, Iran

Source: Annals of Medicine and Surgery Published:2018

Abstract

The knob-associated histidine-rich protein (KAHRP) is an exported parasite protein and the major component of infected erythrocytes by Plasmodium falciparum. P. falciparum histidine-rich protein-1 (PfHRP-1) is docked by KAHRP, which this interaction plays a significant role in cytoadherence of the malaria protozoan to erythrocytes and pathogenicity. The most polymorphic region of the PfHRP-1 is the C-terminal of decapeptide repeat domain (region III). The main objective of this study was to explore the genetic diversity at the region III of KAHRP in P. falciparum isolates from Burundi. In the present study, the nested PCR was performed for the amplification of the coding gene (kahrp gene) for region III in 35 P. falciparum isolates from Burundi. The nested PCR products of seven randomly selected isolates were purified and then sequenced. As the result, three allelic forms (340 bp, 370 bp, and 400 bp) were seen at the C-terminal domain of kahrp gene. The existence of multiple alleles of the kahrp gene revealed the presence of different P. falciparum strains in Burundi. It is suggested that the results could be useful in designing and the improvement of targeted therapy agents for falciparum malaria. © 2018

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Hossein Keshavarz Valian (1)

Style	Citing Format
MLA	Mardani A, et al.. "Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa." Annals of Medicine and Surgery, vol. 34, no. , 2018, pp. 34-38.
APA	Mardani A, Ziaei Hezarjaribi H, Fakhar M, Emadi SN, Rezaeirad A, Butore J, Claudete N (2018). Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa. Annals of Medicine and Surgery, 34(), 34-38.
Chicago	Mardani A, Ziaei Hezarjaribi H, Fakhar M, Emadi SN, Rezaeirad A, Butore J, Claudete N. "Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa." Annals of Medicine and Surgery 34, no. (2018): 34-38.
Harvard	Mardani A et al. (2018) 'Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa', Annals of Medicine and Surgery, 34(), pp. 34-38.
Vancouver	Mardani A, Ziaei Hezarjaribi H, Fakhar M, Emadi SN, Rezaeirad A, Butore J, et al.. Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa. Annals of Medicine and Surgery. 2018;34():34-38.
BibTex	@article{ author = {Mardani A and Ziaei Hezarjaribi H and Fakhar M and Emadi SN and Rezaeirad A and Butore J and Claudete N}, title = {Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa}, journal = {Annals of Medicine and Surgery}, volume = {34}, number = {}, pages = {34-38}, year = {2018} }
RIS	TY - JOUR AU - Mardani A AU - Ziaei Hezarjaribi H AU - Fakhar M AU - Emadi SN AU - Rezaeirad A AU - Butore J AU - Claudete N TI - Genetic Diversity at the C-Terminal Domain of Knob-Associated Histidine-Rich Protein (Kahrp) of Plasmodium Falciparum Isolates From Burundi, Eastern Africa JO - Annals of Medicine and Surgery VL - 34 IS - SP - 34 EP - 38 PY - 2018 ER -

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