Ferulago Angulata As a Good Radioprotector Against Genotoxicity Publisher Pubmed



Moshafi MH¹ ; Torabizadeh SA² ; Mohamadnezhad F¹ ; Jomehzadeh A³ ; Khodaei M⁴ ; Fekri HS^{2, 5} Show Affiliations
Source: Current Radiopharmaceuticals Published:2022

Abstract

Introduction: Natural products can be used as radioprotector agents because of containing phenolic compounds and several flavonoids with antioxidant properties. When the normal cells are exposed to ionizing radiation, they generate free radicals and reactive oxygen species that can cause damage in DNA, which leads to cellular dysfunction or even cell death. However, it is neces-sary to identify new radioprotective agents to protect normal cells. Ferulago angulata (F. angulata), a medicinal plant, can be used as a new radioprotective agent. Purpose: In this study, the radioprotective effect of F. angulata was evaluated against genotoxicity and oxidative stress induced by ionizing radiation in human blood lymphocytes. Methods: The antioxidant activity of F. angulata was assayed using FRAP and DPPH methods. Then, the human blood samples were incubated with F. angulata at different concentrations (25, 50, 100, and 200 μM) and subsequently exposed to IR at a dose of 2Gy. The radioprotective effect of F. angulata on the exposed cells was assessed by the micronucleus (MN) method. Also, bio-markers of oxidative stress in the exposed cells were evaluated by malondialdehyde (MDA) and su-peroxide dismutase (SOD) methods. Results: Our findings showed that F. angulata reduced the frequency of MN induced by IR in exposed cells. At a 200 μM concentration of F. angulata, the maximum reduction in the frequency of MN (63.11%) was observed that demonstrated a high degree of radioprotection. Afterward, pre-treatment at 200 μM concentration of F. angulata inhibited oxidative stress in irradiated lympho-cytes, leading to a reduction in MN frequency and MDA levels while SOD activity was enhanced in the exposed cells. Conclusion: F. angulata as a natural radioprotective agent can protect normal cells against reactive oxygen species and genetic damage induced by IR. © 2022 Bentham Science Publishers.