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Minimizing Cytotoxic Effects on Normal Cells: Enhanced Targeted Delivery of Docetaxel Via Pcl-Peg-Pcl Nanocarriers in Breast Cancer Treatment Publisher



Khojastehfar A ; Mostafazadeh A ; Zabihi E ; Nataj H ; Hakemi P ; Janbabaei G ; Pouramir M
Authors

Source: Journal of Water and Environmental Nanotechnology Published:2025


Abstract

Nanoparticle-based drug delivery systems are rapidly advancing in cancer therapy, providing reduced toxic side effects and improved efficacy through controlled drug release. This study encapsulated docetaxel (DTX) in a PCL-PEG-PCL matrix using a modified nano-precipitation method, focusing on its cytotoxic effects on breast cancer cell lines. The research aimed to clarify the mechanisms behind the cytotoxic effects and cell death induced by DTX-loaded nano-carriers, utilizing human breast cancer cell lines as an experimental model. The synthesized nano-carriers were characterized through methods such as transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). Drug release kinetics and encapsulation efficiency were evaluated with high-performance liquid chromatography (HPLC) and dialysis techniques. The cytotoxicity of DTX-loaded nano-carriers and free DTX was assessed in MCF-7, MDA-MB-231, and fibroblast cell lines over 48 and 72 hours using MTT assays to measure viability and Hoechst 33342 staining for apoptosis. Results showed that nano-carriers significantly inhibited cell growth in MCF-7 and MDA-MB-231 cells, especially with formulation P1 over 72 hours, compared to 48 hours. Apoptosis assays confirmed the effective induction of cell death by nano-carriers. The IC50 values for formulation P1 were substantially higher after 72 hours. Overall, these findings suggest that the designed nano-carriers promote controlled drug release and enhance apoptotic cell death while reducing the toxic side effects of docetaxel on normal cells, highlighting their potential in cancer therapeutics. © 2025, Iranian Environmental Mutagen Society. All rights reserved.
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