The Assessment of Toxicity Characteristics of Cellular Uptake of Paramagnetic Nanoparticles As a New Magnetic Resonance Imaging Contrast Agent Publisher



Moghimi H¹ ; Zohdiaghdam R² ; Riahialam N³ ; Behrouzkia Z⁴ Show Affiliations
Source: Iranian Journal of Pharmaceutical Research Published:2019

Abstract

Nanoparticles are unique that enable many promising medical and technological applications in their physical, and chemical properties. It is widely accepted that nanoparticles should be thoroughly tested for health nanotoxicity, but a moderate risk analysis is currently prevented by a revealing absence of mechanistic knowledge of nanoparticle toxicity. The purpose of this study was to assess in-vitro cytotoxicity of Gadolinium oxide with diethylene glycol polymer (Gd2 O3-DEG) and magneto liposome nanoparticles (MLNs) in Hepa 1-6 cell lines as models to assess nanotoxicity in-vitro. The effects of magnetic nanoparticles on these cell lines were evaluated by light microscopy and standard cytotoxicity assays. The underlying interactions of these nanoparticles with physiological fluids are key characteristics of the perception of their biological efficacy, and these interactions can perhaps be performed to relieve unpleasant toxic effects. Our results demonstrated that the Gd2 O3-DEG and MLNs had significantlydifferent non-cytotoxic effects. Our results suggest that these cell lines provide valuable models to assess the cytotoxicity of nanoparticles in-vitro. The results of the present study demonstrated that MLNs and Gd2 O3-DEG with lower longitudinal relaxation time (T1 ) than Gadolinium Pentetic acid (Gd-DTPA) in Hepa 1-6 cell lines are sensitive positive Magnetic Resonance Imaging (MRI) contrast agents that could be as attractive as candidates for cellular and molecular lipid content targets such as liver diagnostic applications. These data reveal that MLNs is a useful positive contrast agent for targeting and cell tracking. This will help to image of cells and special organs like liver that uptakes liposomal formulation very well. © 2019, Iranian Journal of Pharmaceutical Research. All rights reserved.