Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran

Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran Publisher

Azarsa M¹ ; Ohadian Moghadam S² ; Rahbar M³ ; Baseri Z⁴ ; Pourmand MR⁵

Show Affiliations

1. Department of Microbiology, Khoy University of Medical Sciences, Khoy, Iran
2. Uro-Oncology Research Centre, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Microbiology, Reference Health Laboratories Research Centre, Ministry of Health and Medical Education, Tehran, Iran
4. Central Laboratory of Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: New Microbes and New Infections Published:2019

Abstract

The emergence of penicillin non-susceptible Streptococcus pneumoniae (PNSP) isolates can pose significant challenges to today's health-care system. Resistant clonal isolates are disseminated in different regions and countries, and this study was focused on the description of the epidemiological spread of these strains. Clinical samples were collected from individuals admitted to hospitals affiliated to the Tehran University of Medical Sciences, Iran. To investigate the molecular characteristics of PNSP isolates, they were subjected to molecular typing using multi-locus sequence typing (MLST). Serotype distributions of S. pneumoniae isolates were also evaluated by multiplex PCR assay. The most prevalent serotypes in the PNSP isolates were 23F, 19F, 14, 3 and 9V. Two isolates were considered as a non-vaccine serotype. The MLST analysis showed that PNSP isolates belonged to five different clonal complexes (CC180, CC217, CC81, CC63 and CC320) and 42% (5/12) of the sequence types were novel (12936, 12937, 12938, 12939 and 12940). This study indicates the high level of heterogeneity that is present among PNSP isolates. Unexpected high genetic diversity in small populations indicates consecutive diversification of resistant strains. © 2019 The Authors

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Setareh Mamishi Khanlagh (7)

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Style	Citing Format
MLA	Azarsa M, et al.. "Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran." New Microbes and New Infections, vol. 32, no. , 2019, pp. -.
APA	Azarsa M, Ohadian Moghadam S, Rahbar M, Baseri Z, Pourmand MR (2019). Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran. New Microbes and New Infections, 32(), -.
Chicago	Azarsa M, Ohadian Moghadam S, Rahbar M, Baseri Z, Pourmand MR. "Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran." New Microbes and New Infections 32, no. (2019): -.
Harvard	Azarsa M et al. (2019) 'Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran', New Microbes and New Infections, 32(), pp. -.
Vancouver	Azarsa M, Ohadian Moghadam S, Rahbar M, Baseri Z, Pourmand MR. Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran. New Microbes and New Infections. 2019;32():-.
BibTex	@article{ author = {Azarsa M and Ohadian Moghadam S and Rahbar M and Baseri Z and Pourmand MR}, title = {Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran}, journal = {New Microbes and New Infections}, volume = {32}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Azarsa M AU - Ohadian Moghadam S AU - Rahbar M AU - Baseri Z AU - Pourmand MR TI - Molecular Serotyping and Genotyping of Penicillin Non-Susceptible Pneumococci: The Introduction of New Sequence Types, Tehran, Iran JO - New Microbes and New Infections VL - 32 IS - SP - EP - PY - 2019 ER -

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