Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effectiveness of Activated Sodium Hypochlorite Irrigation by Shock Wave-Enhanced Emission Photoacoustic Streaming, Sonic and Ultrasonic Devices in Removing Enterococcus Faecalis Biofilm From Root Canal System Publisher



Assadian H1 ; Fathollahi S2 ; Pourhajibagher M3 ; Solimei L4 ; Benedicenti S4 ; Chiniforush N4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, 14399-55991, Iran
  2. 2. Private Practice, Tehran, 19479-53893, Iran
  3. 3. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, 14155-5583, Iran
  4. 4. Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Viale Benedetto XV, 6, Genoa, 16132, Italy

Source: Journal of Clinical Medicine Published:2024


Abstract

Aim: To compare shock wave-enhanced emission photoacoustic streaming (SWEEPS) with sonic- and ultrasonically activated irrigation systems in removing Enterococcus faecalis biofilm from the root canal system. Methodology: Fifty human single-canalled mandibular premolars were included in the study. After access cavity preparation, the root canals were prepared to a standardized size and taper. Then, the entire root surface was covered with two layers of resin, and the root apices were sealed before sterilization. All root canals were inoculated with E. faecalis biofilm, and the samples were incubated aerobically for 2 weeks at 37 °C. Biofilm formation was confirmed by scanning electron microscopy. All samples were randomly divided into five groups (n = 10 each) based on their irrigation activation method as A (no treatment or negative control), B (no irrigation or positive control), C (sonically activated irrigation (SAI)), D (ultrasonically activated irrigation (UAI)), and E (needle irrigation activated by an Er: YAG laser device using a SWEEPS quartz tip (SWEEPS)). Then, dentine chips were retrieved, vortexed, and diluted for colony-forming unit counts. Data were analysed using analysis of variance and post-hoc Tukey tests (α = 5%). Results: All methods could significantly reduce E. faecalis biofilm compared with control so that the UAI, SWEEPS, and SAI groups indicated a 23.54%, 14.89%, and 7.81% biofilm reduction, respectively. UAI demonstrated a significantly more effective reduction of E. faecalis biofilm than SAI (p = 0.004). Conclusions: All irrigation activation methods significantly reduced E. faecalis biofilm, with ultrasonic use being the most effective. © 2024 by the authors.
Related Docs
View other Related Docs
1. Effect of Sweeps and Pips Techniques on Dye Extrusion in Photodynamic Therapy Procedure After Root Canal Preparation, Photodiagnosis and Photodynamic Therapy (2023)
2. Comparative Effects of the Conventional, Ultrasonic, and Laser-Activated Irrigation on Penetration Depth of Three Photosensitizers in the Root Canal System, Photodiagnosis and Photodynamic Therapy (2024)
3. In Vitro Effects of Laser-Activated Irrigation Methods on Apical Extrusion of Indocyanine Green Mediated Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2025)
4. Comparative Effects of Sweeps Technique and Antimicrobial Photodynamic Therapy by Using Curcumin and Nano-Curcumin on Enterococcus Faecalis Biofilm in Root Canal Treatment, Photodiagnosis and Photodynamic Therapy (2022)
5. Clinical Approach of High Technology Techniques for Control and Elimination of Endodontic Microbiota, Journal of Lasers in Medical Sciences (2015)
6. Sweeps-Assisted Antibacterial Photodynamic Therapy Against Dual-Species Biofilms in Mandibular Molars: An in Vitro Study, Pharmaceuticals (2025)
7. In Vitro Assessment of Sweeps and Antimicrobial Photodynamic Therapy Alone or in Combination for Eradicating Enterococcus Faecalis Biofilm in Root Canals, Pharmaceutics (2023)
8. The Evaluation of Sweeps Plus Antimicrobial Photodynamic Therapy With Indocyanine Green in Eliminating Enterococcus Faecalis Biofilm From Infected Root Canals: An in Vitro Study, Biomedicines (2023)
Experts (# of related papers)
View all Related Experts
Maryam Pourhajibagher (11)
Abbas Bahador (11)
Other Related Docs
9. Efficacy of the Orifice-Level Passive Ultrasonic Activation Technique in Removing Debris From Mandibular Molars: An Ex Vivo Study, Journal of Lasers in Medical Sciences (2024)
10. Comparison of Antibacterial Effect of Four Irrigation Solutions in Primary Root Canal Infections: A Clinical Study, Iranian Endodontic Journal (2018)
11. Comparative Efficacy of Er:Yag Laser and Shock Wave Enhanced Emission Photoacoustic Streaming (Sweeps) for Smear Layer Removal: An Ex Vivo Study, World Journal of Dentistry (2023)
12. The Evaluation of Cultivable Microbiota Profile in Patients With Secondary Endodontic Infection Before and After Photo-Activated Disinfection, Photodiagnosis and Photodynamic Therapy (2017)
13. Entrococcus Faecalis Elimination in Root Canals Using Silver Nanoparticles, Photodynamic Therapy, Diode Laser, or Laser-Activated Nanoparticles: An In Vitro Study, Journal of Endodontics (2017)
14. Ex Vivo Comparison of Antibacterial Efficacy of Conventional Chemomechanical Debridement Alone and in Combination With Light-Activated Disinfection and Laser Irradiation Against Enterococcus Faecalis Biofilm, Photodiagnosis and Photodynamic Therapy (2020)
15. An in Vivo Evaluation of Microbial Diversity Before and After the Photo-Activated Disinfection in Primary Endodontic Infections: Traditional Phenotypic and Molecular Approaches, Photodiagnosis and Photodynamic Therapy (2018)
16. The in Vitro Antibacterial Efficacy of Persian Green Tea Extract As an Intracanal Irrigant on Enterococcus Faecalis Biofilm, Iranian Endodontic Journal (2016)
17. The Antibacterial Activity of an Epoxy Resin-Based Dental Sealer Containing Bioactive Glass, Hydroxyapatite, and Fluorohydroxyapatite Nanoparticles Against Enterococcus Faecalis and Streptococcus Mitis, Nanomedicine Journal (2020)
18. Effect of Ultrasonic Activation on the Efficacy of Antimicrobial Photodynamic Therapy: Evaluation of Penetration Depth of Photosensitizer and Elimination of Enterococcus Faecalis Biofilms, Photodiagnosis and Photodynamic Therapy (2019)
19. Modulation of Virulence in Enterococcus Faecalis Cells Surviving Antimicrobial Photodynamic Inactivation With Reduced Graphene Oxide-Curcumin: An Ex Vivo Biofilm Model, Photodiagnosis and Photodynamic Therapy (2020)
20. Antimicrobial Photodynamic Therapy Assessment of Three Indocyanine Green-Loaded Metal-Organic Frameworks Against Enterococcus Faecalis, Photodiagnosis and Photodynamic Therapy (2018)
21. In Vitro and Ex Vivo Antimicrobial Efficacy of Nano-Mgo in the Elimination of Endodontic Pathogens, Clinical Oral Investigations (2015)
22. Penetration Depth of Sodium Hypochlorite in Dentinal Tubules After Conventional Irrigation, Passive Ultrasonic Agitation and Nd: Yag Laser Activated Irrigation, Journal of Lasers in Medical Sciences (2016)
23. Can Antimicrobial Photodynamic Therapy (Apdt) Enhance the Endodontic Treatment?, Journal of Lasers in Medical Sciences (2016)
24. Dual Wavelength Irradiation Antimicrobial Photodynamic Therapy Using Indocyanine Green and Metformin Doped With Nano-Curcumin As an Efficient Adjunctive Endodontic Treatment Modality, Photodiagnosis and Photodynamic Therapy (2020)
25. Effect of Er:Yag Laser Irradiation Using Sweeps and Pips Technique on Dye Penetration Depth After Root Canal Preparation, Photodiagnosis and Photodynamic Therapy (2021)
26. Photodynamic Inactivation of Endopathogenic Microbiota Using Curcumin-Mediated Antimicrobial Photodynamic Therapy, Journal of Sciences# Islamic Republic of Iran (2018)
27. Culture-Dependent Approaches to Explore the Prevalence of Root Canal Pathogens From Endodontic Infections, Brazilian Oral Research (2017)
28. Bacterial Microleakage in Endodontically Treated Teeth Following Two Methods of Postspace Preparation at Two-Time Intervals: An in Vitro Study, Journal of Indian Prosthodontic Society (2022)
29. Detection of Enterococcus Faecalis and the Red Complex Bacteria Analyzed by the Checkerboard Technique for Dna-Dna Hybridization in Endodontic Infections: A Systematic Review and Meta-Analysis, Diagnostic Microbiology and Infectious Disease (2025)
30. Evaluation of the Antibacterial Efficacy of Various Root Canal Disinfection Methods Against Enterococcus Faecalis Biofilm. an Ex-Vivo Study, Photodiagnosis and Photodynamic Therapy (2018)
31. Antibacterial Efficacy of Antibiotic Pastes Versus Calcium Hydroxide Intracanal Dressing: A Systematic Review and Meta-Analysis of Ex Vivo Studies, Journal of Conservative Dentistry (2022)