Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines

Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines Publisher Pubmed

Jazayeri SH^{1, 2} ; Amiriyekta A² ; Bahrami S² ; Gourabi H² ; Sanati MH^{2, 3} ; Khorramizadeh MR⁴

Show Affiliations

1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Genetics, Reproductive Biomedicine Research Center, ACECR, Royan Institute for Reproductive Biomedicine, P.O. Box: 14155-6343, Tehran, Iran
3. Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
4. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, P.O. Box: 1411413137, Tehran, Iran

Source: Applied Biochemistry and Biotechnology Published:2018

Abstract

The rapid growth of global biopharmaceutical market in the recent years has been a good indication of its significance in biotechnology industry. During a long period of time in recombinant protein production from 1980s, optimizations in both upstream and downstream processes were launched. In this regard, one of the most promising strategies is expression vector engineering technology based on incorporation of DNA opening elements found in the chromatin border regions of vectors as well as targeting gene integration. Along with these approaches, cell line engineering has revealed convenient outcomes in isolating high-producing clones. According to the fact that more than 50% of the approved therapeutic proteins is being manufactured in mammalian cell lines, in this review, we focus on several approaches and developments in vector and cell line engineering technologies in mammalian cell culture. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

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Mohammad Reza Khorramizadeh (1)

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Style	Citing Format
MLA	Jazayeri SH, et al.. "Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines." Applied Biochemistry and Biotechnology, vol. 185, no. 4, 2018, pp. 986-1003.
APA	Jazayeri SH, Amiriyekta A, Bahrami S, Gourabi H, Sanati MH, Khorramizadeh MR (2018). Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines. Applied Biochemistry and Biotechnology, 185(4), 986-1003.
Chicago	Jazayeri SH, Amiriyekta A, Bahrami S, Gourabi H, Sanati MH, Khorramizadeh MR. "Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines." Applied Biochemistry and Biotechnology 185, no. 4 (2018): 986-1003.
Harvard	Jazayeri SH et al. (2018) 'Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines', Applied Biochemistry and Biotechnology, 185(4), pp. 986-1003.
Vancouver	Jazayeri SH, Amiriyekta A, Bahrami S, Gourabi H, Sanati MH, Khorramizadeh MR. Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines. Applied Biochemistry and Biotechnology. 2018;185(4):986-1003.
BibTex	@article{ author = {Jazayeri SH and Amiriyekta A and Bahrami S and Gourabi H and Sanati MH and Khorramizadeh MR}, title = {Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines}, journal = {Applied Biochemistry and Biotechnology}, volume = {185}, number = {4}, pages = {986-1003}, year = {2018} }
RIS	TY - JOUR AU - Jazayeri SH AU - Amiriyekta A AU - Bahrami S AU - Gourabi H AU - Sanati MH AU - Khorramizadeh MR TI - Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines JO - Applied Biochemistry and Biotechnology VL - 185 IS - 4 SP - 986 EP - 1003 PY - 2018 ER -

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