Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa)

Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa) Publisher Pubmed

Salmaninejad A¹ ; Estiar MA¹ ; Gill RK² ; Shih JH³ ; Hewitt S⁴ ; Jeon HS⁵ ; Fukuoka J⁶ ; Shilo K⁷ ; Shakoori A^{1, 2, 3} ; Jen J⁸

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1. Department of Medical Genetics, Cancer Institute of Iran, Tehran University of Medical Sciences, Keshavarz Blvd., Tehran, 13145-158, Iran
2. Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, Bethesda, MD, United States
3. National Cancer Institute, Executive Boulevard, Bethesda, MD, United States
4. Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States
5. Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, Daegu, South Korea
6. Department of Pathology, Nagasaki University, Graduate School of Biomedical Sciences, Sakamoto, Nagasaki, Japan
7. Department of Pulmonary and Mediastinal Pathology, Armed Forces Institute of Pathology, Washington, DC, United States
8. Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, United States

Source: Clinical Laboratory Published:2015

Abstract

Background: Immunohistochemical analysis (IHC) of tissue microarray (TMA) slides enables large sets of tissue samples to be analyzed simultaneously on a single slide. However, manual evaluation of small cores on a TMA slide is time consuming and error prone. Methods: We describe a computer aided scoring and analysis (CASA) method to allow facile and reliable scoring of IHC staining using TMA containing 300 non-small cell lung cancer (NSCLC) cases. In the two previous published papers utilizing our TMA slides of lung cancer we examined 18 proteins involved in the chromatin machinery. We developed our study using more proteins of the chromatin complex and several transcription factors that facilitate the chromatin machinery. Then, a total of 78 antibodies were evaluated by CASA to derive a normalized intensity value that correlated with the overall staining status of the targeting protein. The intensity values for TMA cores were then examined for association to clinical variables and predictive significance individually and with other factors. Results: Using our TMA, the intensity of several protein pairs were significantly correlated with an increased risk of death in NSCLC. These included c-Myc with p16, mSin3A with p16 and c-Myc with mSin A. Predictive values of these pairs remained significant when evaluated based on standard IHC scores. Conclusions: Our results demonstrate the usefulness of CASA as a valuable tool for systematic assessment of TMA slides to identify potential predictive biomarkers using a large set of primary human tissues.

Style	Citing Format
MLA	Salmaninejad A, et al.. "Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa)." Clinical Laboratory, vol. 61, no. 5-6, 2015, pp. 549-559.
APA	Salmaninejad A, Estiar MA, Gill RK, Shih JH, Hewitt S, Jeon HS, Fukuoka J, Shilo K, Shakoori A, Jen J (2015). Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa). Clinical Laboratory, 61(5-6), 549-559.
Chicago	Salmaninejad A, Estiar MA, Gill RK, Shih JH, Hewitt S, Jeon HS, Fukuoka J, Shilo K, Shakoori A, Jen J. "Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa)." Clinical Laboratory 61, no. 5-6 (2015): 549-559.
Harvard	Salmaninejad A et al. (2015) 'Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa)', Clinical Laboratory, 61(5-6), pp. 549-559.
Vancouver	Salmaninejad A, Estiar MA, Gill RK, Shih JH, Hewitt S, Jeon HS, et al.. Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa). Clinical Laboratory. 2015;61(5-6):549-559.
BibTex	@article{ author = {Salmaninejad A and Estiar MA and Gill RK and Shih JH and Hewitt S and Jeon HS and Fukuoka J and Shilo K and Shakoori A and Jen J}, title = {Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa)}, journal = {Clinical Laboratory}, volume = {61}, number = {5-6}, pages = {549-559}, year = {2015} }
RIS	TY - JOUR AU - Salmaninejad A AU - Estiar MA AU - Gill RK AU - Shih JH AU - Hewitt S AU - Jeon HS AU - Fukuoka J AU - Shilo K AU - Shakoori A AU - Jen J TI - Expression Analysis of P16, C-Myc, and Msin3a in Non-Small Cell Lung Cancer by Computer Aided Scoring and Analysis (Casa) JO - Clinical Laboratory VL - 61 IS - 5-6 SP - 549 EP - 559 PY - 2015 ER -

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