Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method

Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method Publisher

Karimi S¹ ; Bahrami N^{2, 3} ; Sharifi K⁴ ; Daustany M⁵ ; Baghbaniarani F¹ ; Kazempour M⁶ ; Mohamadnia A^{4, 7}

Show Affiliations

1. Department of Genetics and Biotechnology, Varamin-Pishva branch, Islamic Azad University, Varamin, Iran
2. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran
6. Biostatistics Department, Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Iran
7. Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, 1956944413, Iran

Source: Minerva Pneumologica Published:2017

Abstract

BACKGROUND: Lung cancer is one of the most common malignant diseases in the world that has turned to into a big problem for world health organizations in the diagnosis and cure field. Investigating the cancer related genes expression can have a significant role in early diagnosis and determining the kind of cancer. However, there is no specific biomarker to aid early clinical diagnosis. In this study, gene expression level of MUC1 and CEA were investigated among the non-small cell lung cancer (NSCLC) patients. METHODS: In this study, 40 NSCLC patients were compared with 40 healthy people. Real-time RT-PCR method was used to determine the expression level of MUC1 mRNA and CEA mRNA biomarkers in pleural fluid of patients and healthy people. RESULTS: Comparison of these groups with t-test showed no meaningful difference regarding age average. Positivity of CEA mRNA marker in NSCLC patients were observed in 30 of 40 patients and this marker's sensitivity was determined at 75%. In the healthy group, 11 of 40 had positive marker. MUC1 mRNA marker among patients were positive in 28 of them which shows 70% sensitivity and it was positive in 7 out of 40 people in the healthy group. CONCLUSIONS: The results of this study can be considered a diagnosing-screening test for early discovery of the disease since MUC1 mRNA and CEA mRNA markers were evaluated at good sensitivity levels. For further validation of the research results, it is suggested to perform a more widespread study with more samples. © 2017 Edizioni Minerva Medica.

2. Expression of Ck19-Mrna and Cea-Mrna Biomarkers in Pleural Fluid of Patients With Non-Small Cell Lung Cancer, Minerva Pneumologica (2017)

3. Detection of Vascular Endothelial Growth Factor Messenger Rna (Vegf Mrna), Cytokeratin-19 Mrna (Ck19 Mrna), and Vascular Endothelial Growth Factor (Vegf) Protein Biomarker in the Peripheral Blood of Patients With Breast Cancer, Journal of Isfahan Medical School (2017)

4. Predictive Molecular Blood Biomarkers in Non-Small Cell Lung Cancer, Journal of Cellular and Molecular Anesthesia (2022)

5. Diagnostic Value of Lunx Mrna and Cea Mrna Expression in Pleural Fluid of Patients With Non-Small Cell Lung Cancer, Minerva Pneumologica (2017)

6. Investigating Pik3r3 and Atp2a1 Genes Expressions in Ventilator-Associated Pneumonia Patients Admitted to the Intensive Care Unit of Masih Daneshvari Hospital in 2016, Reports of Biochemistry and Molecular Biology (2018)

7. Multi-Gene Expression in Anthracosis of the Lungs As One of the Risk Factors for Non-Small Cell Lung Cancer, Asian Pacific Journal of Cancer Prevention (2017)

8. Crosstalk Between Muc1 and Vegf in Angiogenesis and Metastasis: A Review Highlighting Roles of the Muc1 With an Emphasis on Metastatic and Angiogenic Signaling, Cancer Cell International (2021)

Experts (# of related papers)

Naghmeh Bahrami (7)

Style	Citing Format
MLA	Karimi S, et al.. "Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method." Minerva Pneumologica, vol. 56, no. 1, 2017, pp. 18-24.
APA	Karimi S, Bahrami N, Sharifi K, Daustany M, Baghbaniarani F, Kazempour M, Mohamadnia A (2017). Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method. Minerva Pneumologica, 56(1), 18-24.
Chicago	Karimi S, Bahrami N, Sharifi K, Daustany M, Baghbaniarani F, Kazempour M, Mohamadnia A. "Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method." Minerva Pneumologica 56, no. 1 (2017): 18-24.
Harvard	Karimi S et al. (2017) 'Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method', Minerva Pneumologica, 56(1), pp. 18-24.
Vancouver	Karimi S, Bahrami N, Sharifi K, Daustany M, Baghbaniarani F, Kazempour M, et al.. Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method. Minerva Pneumologica. 2017;56(1):18-24.
BibTex	@article{ author = {Karimi S and Bahrami N and Sharifi K and Daustany M and Baghbaniarani F and Kazempour M and Mohamadnia A}, title = {Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method}, journal = {Minerva Pneumologica}, volume = {56}, number = {1}, pages = {18-24}, year = {2017} }
RIS	TY - JOUR AU - Karimi S AU - Bahrami N AU - Sharifi K AU - Daustany M AU - Baghbaniarani F AU - Kazempour M AU - Mohamadnia A TI - Investigating Gene Expression Level of Muc1 and Cea in Pleural Fluid of Nsclc Lung Cancer Patients With Real-Time Rt-Pcr Method JO - Minerva Pneumologica VL - 56 IS - 1 SP - 18 EP - 24 PY - 2017 ER -

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