Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Assessment of Key Elements in the Innate Immunity System Among Patients With Hiv, Hcv, and Coinfections of Hiv/Hcv Publisher Pubmed



Moradi M1 ; Tabibzadeh A1 ; Javanmard D1, 2 ; Ghorbani S1, 3 ; Bokharaeisalim F1, 4 ; Keivani H1 ; Khazeni M5 ; Monavari SHR1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
  3. 3. Student Research Committee, Iran University of Medical Sciences, Tehran, Iran
  4. 4. HIV Laboratory of National Center, Deputy of Health, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Virology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Current HIV Research Published:2020


Abstract

Background: Coinfection of Hepatitis C virus (HCV) with human immunodeficiency virus (HIV) has a higher risk of mortality than HCV or HIV monoinfection. HCV and HIV infections are specified by systemic inflammation, but the inflammation process in HCV/HIV coinfection is much complicated and is not well characterized. Objective: The aim of this study was to analyze the expression of TLR-3, TLR-7, IL-10, IFN-1 (IFN-α, IFN-β), and TNF-α in HIV, HCV and HIV/HCV co-infected patients. Methods: Forty-five patients including HIV group (n=15), HCV group (n=15), HIV/HCV coinfection group (n=15) and healthy control group (n=15) participated. Peripheral blood mononuclear cells (PBMCs) were obtained. PBMC-RNA, HCV and HIV RNA were extracted from all subjects and cDNA was synthesized. The viral load analyzed by reverse transcription-quantitative PCR (RT-qPCR), and the expression levels of IFN-α, IFN-β, TLR-3, TLR-7, TNF, and IL-10 mRNA were quantified in PBMCs. Results: The levels of IFN-I, IL-10, and TNF-α were overexpressed in all patients’ groups (p<0.05), TLR-7 was upregulated in all groups, but this upregulation was not statistically significant (p>0.05). TLR-3 showed a decrease in all patient groups (p<0.05). The statistical analysis demonstrated that TLR-3 has a negative correlation with HIV load, whereas other genes positively correlated with HIV load. In addition, TLR-3, TNF-α, and IFN-I were negatively correlated with HCV load, whereas TLR-7 and IL-10 s were positively correlated with HCV load. Conclusion: Our results showed a significant relationship between the expression level of innate immunity genes and inflammation in HCV, HIV, and HIV/HCV coinfected patients. © 2020 Bentham Science Publishers.
Related Docs
View other Related Docs
1. Elevated Serum Levels of Adiponectin and Interlukin-28B After Ifn/Rib Therapy in Hepatitis C Virus-Infected Patients, Journal of Infection in Developing Countries (2019)
2. The Presence of Autoantibodies to Cytoplasmic Rod and Ring Particles in the Serum of Patients With Chronic Hepatitis C Virus Infection, Hepatitis Monthly (2016)
3. Recessive Inborn Errors of Type I Ifn Immunity in Children With Covid-19 Pneumonia, Journal of Experimental Medicine (2022)
4. Important Risk Factors of Liver Cirrhosis in Hiv and Hepatitis C Coinfected Patients: A Systematic Review, Open AIDS Journal (2024)
5. Tlr9-Based Immunotherapy for the Treatment of Allergic Diseases, Immunotherapy (2017)
6. Toll-Like Receptors As Targets for Allergen Immunotherapy, Current Opinion in Allergy and Clinical Immunology (2015)
7. Epstein-Barr Virus and Risk of Breast Cancer: A Systematic Review and Meta-Analysis, Future Oncology (2019)
8. Evaluation of Immunological Interaction Between Spermatozoa and Fallopian Tube Epithelial Cells, Andrologia (2015)
Experts (# of related papers)
View all Related Experts
Nima Rezaei (6)
Saeid Amanpour (1)
Other Related Docs
9. Toll-Like Receptors in Atopic Dermatitis: Pathogenesis and Therapeutic Implications, Heliyon (2025)
10. Micrornas 29, 150, 155, 223 Level and Their Relation to Viral and Immunological Markers in Hiv-1 Infected Naive Patients, Future Virology (2018)
11. X-Linked Recessive Tlr7 Deficiency in ~1% of Men Under 60 Years Old With Life-Threatening Covid-19, Science Immunology (2021)
12. Microrna Let-7 and Viral Infections: Focus on Mechanisms of Action, Cellular and Molecular Biology Letters (2022)
13. Association of Hcv Genotype With Viral Load Among Iranian Blood Donors: A Penalized Logistic Regression, Medical Journal of the Islamic Republic of Iran (2019)
14. A Review of Covid-19: The Main Ways of Transmission and Some Prevention Solutions, Clinical Symptoms, More Vulnerable Human Groups, Risk Factors, Diagnosis, and Treatment, Journal of Environmental Treatment Techniques (2020)
15. Preventive Effect of Mitoq Supplementation and Endurance Training on Glioblastoma and Its Consequences: Tlr4/Creb/ Nf-Κβ /Il-1Β Pathway and Behaviors, International Immunopharmacology (2025)
16. Endosomal Toll-Like Receptors Intermediate Negative Impacts of Viral Diseases, Autoimmune Diseases, and Inflammatory Immune Responses on the Cardiovascular System, Expert Review of Clinical Immunology (2025)
17. Defects in Innate Immunity: Receptors and Signaling Components, Encyclopedia of Infection and Immunity (2022)
18. Epilepsy and Innate Immune System: A Possible Immunogenic Predisposition and Related Therapeutic Implications, Human Vaccines and Immunotherapeutics (2015)
19. Mesenchymal Stromal Cells Induce Inhibitory Effects on Hepatocellular Carcinoma Through Various Signaling Pathways, Cancer Cell International (2019)
20. Bifidobacterium Animalis in Combination With Human Origin of Lactobacillus Plantarum Ameliorate Neuroinflammation in Experimental Model of Multiple Sclerosis by Altering Cd4+ T Cell Subset Balance, Biomedicine and Pharmacotherapy (2017)
21. Human Genetic and Immunological Determinants of Critical Covid-19 Pneumonia, Nature (2022)
22. The Role of Toll-Like Receptors in Neuropsychiatric Disorders: Immunopathology, Treatment, and Management, Medicinal Research Reviews (2024)