Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Investigating the Effects of Electrical Stimulation Via Gold Nanoparticles on in Vitro Neurite Outgrowth: Perspective to Nerve Regeneration Publisher



Adel M1, 2, 3 ; Zahmatkeshan M1, 4 ; Johari B5 ; Kharrazi S1 ; Mehdizadeh M6 ; Bolouri B1, 7 ; Rezayat SM1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Deputy of Research and Technology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Nanotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Neuroscience Research Center (NRC), Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Medical Biotechnology, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Cellular and Molecular Research Center, School of Advanced Technologies in Medicine, Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Microelectronic Engineering Published:2017


Abstract

Neural tissue engineering research field has been progressed by using different approaches especially for repairing of damaged neural cells. In addition, it is known that electrical stimulation can be used for neurite growth and nerve regeneration. In this study conductive properties of gold nanoparticles (GNPs, 39 nm) and their contribution to the enhancement of electrical stimulation to nerve cells have been conducted. In experimental section, polyethyleneimine (PEI) polymer coated cover glasses was used to create a positively charged glass surface and adsorption of GNPs was used in conjugation with this polymer coated substrate. Subsequently, PC12 cells were cultured on the modified glass surface and pulsed electric field of 1.5 V, 20 Hz was applied as electrical stimulation for 55 min duration. Images from FESEM showed a uniform distribution of GNPs on glasses surface. In addition, enhanced neurite outgrowth (120 μm) using electrical stimulation was determined by inverted phase contrast microscopy images. Altogether, synergist combination of GNPs together with pulsed electrical stimulation can be used for enhanced nerve regeneration. © 2017 Elsevier B.V.
Related Docs
1. Electroconductive Scaffolds for Tissue Regeneration: Current Opportunities, Pitfalls, and Potential Solutions, Materials Research Bulletin (2021)
2. Conductive Biomaterials As Nerve Conduits: Recent Advances and Future Challenges, Applied Materials Today (2020)
3. Improving Motor Neuron-Like Cell Differentiation of Henscs by the Combination of Epothilone B Loaded Pcl Microspheres in Optimized 3D Collagen Hydrogel, Scientific Reports (2021)
4. Sequential Application of Mineralized Electroconductive Scaffold and Electrical Stimulation for Efficient Osteogenesis, Journal of Biomedical Materials Research - Part A (2018)
5. Using Gold Nanoparticles in Diagnosis and Treatment of Melanoma Cancer, Artificial Cells# Nanomedicine and Biotechnology (2018)
6. Gold Nanorods Reinforced Silk Fibroin Nanocomposite for Peripheral Nerve Tissue Engineering Applications, International Journal of Biological Macromolecules (2019)
7. Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering, Biomolecules (2019)
8. Synergistic Effects of Conductive Pva/Pedot Electrospun Scaffolds and Electrical Stimulation for More Effective Neural Tissue Engineering, European Polymer Journal (2020)
Experts (# of related papers)
View all Related Experts
Zahra Hassannejad (1)
Somayeh Ebrahimi Barough (1)