Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Synergistic Effects of Conductive Pva/Pedot Electrospun Scaffolds and Electrical Stimulation for More Effective Neural Tissue Engineering Publisher



Babaie A1, 2 ; Bakhshandeh B3 ; Abedi A1 ; Mohammadnejad J1 ; Shabani I4 ; Ardeshirylajimi A5 ; Reza Moosavi S6 ; Amini J7 ; Tayebi L8
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences and Technology, University of Tehran, Tehran, Iran
  2. 2. Department of Chemistry and Biotechnology, Faculty of Science Engineering and Technology, Swinburne University of Technology, Hawthorn, 3122, Victoria, Australia
  3. 3. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
  4. 4. Department of Biomedical Engineering, Amir Kabir University of Technology, Tehran, Iran
  5. 5. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Mechanical Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran
  8. 8. Marquette University, School of Dentistry, Milwaukee, 53201, WI, United States

Source: European Polymer Journal Published:2020


Abstract

Fabrication and optimization of conductive scaffolds capable of inducing proper intercellular connections through electrical signals is critical for neural tissue engineering. In this research, electrospun conductive PVA (Polyvinyl alcohol)/PEDOT(poly(3,4-ethylenedioxythiophene)) scaffolds were fabricated in different compositions. Conductivity of electrospinning solutions and electrospun scaffolds were measured. Morphology and topography, mechanical properties and water contact angle of scaffolds were analyzed. Chemistry of scaffolds were studied using FTIR analysis, while biocompatibility and cellular interactions with scaffolds were tested using MTT assay and cellular attachment and spreading testing. Our results show improvements in PEDOT-containing scaffolds, in terms of physiochemical properties, and cell viability compared to pure PVA scaffolds. After optimization of scaffolds, real-time PCR analysis was used to study neural differentiation of rat mesenchymal stem cells (MSCs). Scaffold samples with and without induction of electrical stimulation are shown to upregulate β-tubulin, nestin and enolase as compared to TCP samples. Additionally, expression of nestin gene in scaffold samples with electrical stimulation was 1.5 times more significant than scaffold sample. Overall, this study shows that using PVA/PEDOT conductive scaffolds with electrical stimulation can improve cellular response and neural differentiation through mimicking the properties of native neural tissue. © 2020 Elsevier Ltd
Related Docs
View other Related Docs
1. Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering, Biomolecules (2019)
2. Conductive Biomaterials As Nerve Conduits: Recent Advances and Future Challenges, Applied Materials Today (2020)
3. Electroconductive Scaffolds for Tissue Regeneration: Current Opportunities, Pitfalls, and Potential Solutions, Materials Research Bulletin (2021)
4. Concurrent Application of Conductive Biopolymeric Chitosan/ Polyvinyl Alcohol/ Mwcnts Nanofibers, Intracellular Signaling Manipulating Molecules and Electrical Stimulation for More Effective Cardiac Tissue Engineering, Materials Chemistry and Physics (2021)
5. Nanomaterials Supported by Polymers for Tissue Engineering Applications: A Review, Heliyon (2022)
6. Electrical Stimulation of Somatic Human Stem Cells Mediated by Composite Containing Conductive Nanofibers for Ligament Regeneration, Biologicals (2017)
7. 3D Printing in Oral & Maxillofacial Surgery, 3D Printing in Oral & Maxillofacial Surgery (2021)
8. Sequential Application of Mineralized Electroconductive Scaffold and Electrical Stimulation for Efficient Osteogenesis, Journal of Biomedical Materials Research - Part A (2018)
Experts (# of related papers)
View all Related Experts
Somayeh Ebrahimi Barough (4)
Mahmoud Azami (3)
Other Related Docs
9. Naturally Occurring Biological Macromolecules-Based Hydrogels: Potential Biomaterials for Peripheral Nerve Regeneration, International Journal of Biological Macromolecules (2020)
10. Electric-Responsive Materials: Properties, Design, and Applications, ACS Symposium Series (2023)
11. Development of Polyvinyl Alcohol Fibrous Biodegradable Scaffolds for Nerve Tissue Engineering Applications: In Vitro Study, International Journal of Polymeric Materials and Polymeric Biomaterials (2015)
12. A Novel Electroactive Agarose-Aniline Pentamer Platform As a Potential Candidate for Neural Tissue Engineering, Scientific Reports (2017)
13. Enhanced Growth and Differentiation of Neural Stem Cells on Alginate/ Collagen/Reduced Graphene Oxide Composite Hydrogel Incorporated With Lithium Chloride, BioImpacts (2023)
14. Tailoring Synthetic Polymeric Biomaterials Towards Nerve Tissue Engineering: A Review, Artificial Cells# Nanomedicine and Biotechnology (2019)
15. Biomaterials Selection for Neuroprosthetics, Current Opinion in Biomedical Engineering (2018)
16. A Facile Route to the Synthesis of Anilinic Electroactive Colloidal Hydrogels for Neural Tissue Engineering Applications, Journal of Colloid and Interface Science (2018)
17. Tissue Engineering Scaffolds in the Treatment of Brain Disorders in Geriatric Patients, Artificial Organs (2019)
18. Appropriate Scaffold Selection for Cns Tissue Engineering, Avicenna Journal of Medical Biotechnology (2020)
19. Preparation of Fibrin Gel Scaffolds Containing Mwcnt/Pu Nanofibers for Neural Tissue Engineering, Journal of Biomedical Materials Research - Part A (2019)
20. Interplay Between Angiogenesis and Neurogenesis in Nerve Regeneration, Biomaterials for Vasculogenesis and Angiogenesis (2022)
21. Biodegradable Functional Macromolecules As Promising Scaffolds for Cardiac Tissue Engineering, Polymers for Advanced Technologies (2022)
22. Neuroregenerative Effects of Olfactory Ensheathing Cells Transplanted in a Multi-Layered Conductive Nanofibrous Conduit in Peripheral Nerve Repair in Rats, Journal of Biomedical Science (2015)
23. Influence of Oriented Nanofibrous Pcl Scaffolds on Quantitative Gene Expression During Neural Differentiation of Mouse Embryonic Stem Cells, Journal of Biomedical Materials Research - Part A (2016)
24. Recent Advances in Enhances Peripheral Nerve Orientation: The Synergy of Micro or Nano Patterns With Therapeutic Tactics, Journal of Nanobiotechnology (2024)
25. Gold Nanorods Reinforced Silk Fibroin Nanocomposite for Peripheral Nerve Tissue Engineering Applications, International Journal of Biological Macromolecules (2019)
26. Electroactive Bio-Epoxy Incorporated Chitosan-Oligoaniline As an Advanced Hydrogel Coating for Neural Interfaces, Progress in Organic Coatings (2019)
27. Injectable Conductive Nanocomposite Hydrogels for Cardiac Tissue Engineering: Focusing on Carbon and Metal-Based Nanostructures, European Polymer Journal (2022)
28. Application of Electrospun Nanofibrous Phbv Scaffold in Neural Graft and Regeneration: A Mini-Review, Nanomedicine Research Journal (2016)
29. Investigating the Effects of Electrical Stimulation Via Gold Nanoparticles on in Vitro Neurite Outgrowth: Perspective to Nerve Regeneration, Microelectronic Engineering (2017)
30. Engineering the Niche for Hair Regeneration — a Critical Review, Nanomedicine: Nanotechnology# Biology# and Medicine (2019)
31. In Vitro Effect of Graphene Structures As an Osteoinductive Factor in Bone Tissue Engineering: A Systematic Review, Journal of Biomedical Materials Research - Part A (2018)
32. Cellular Activity of Wharton's Jelly-Derived Mesenchymal Stem Cells on Electrospun Fibrous and Solvent-Cast Film Scaffolds, Journal of Biomedical Materials Research - Part A (2016)
33. Drug Release, Cell Adhesion and Wound Healing Evaluations of Electrospun Carboxymethyl Chitosan/Polyethylene Oxide Nanofibres Containing Phenytoin Sodium and Vitamin C, IET Nanobiotechnology (2015)
34. Advanced Approaches to Regenerate Spinal Cord Injury: The Development of Cell and Tissue Engineering Therapy and Combinational Treatments, Biomedicine and Pharmacotherapy (2022)
35. Improved Cellular Response on Functionalized Polypyrrole Interfaces, Journal of Cellular Physiology (2019)
36. Protein Adsorption on Polymers, Materials Today Communications (2018)
37. Synthesis, Characterization and Performance Enhancement of Dry Polyaniline-Coated Neuroelectrodes for Electroencephalography Measurement, Current Applied Physics (2021)
38. A Novel Polycaprolactone/Carbon Nanofiber Composite As a Conductive Neural Guidance Channel: An in Vitro and in Vivo Study, Progress in Biomaterials (2019)
39. Rapid Induction of Neural Differentiation in Human Umbilical Cord Matrix Mesenchymal Stem Cells by Camp-Elevating Agents, International Journal of Molecular and Cellular Medicine (2016)