Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches

Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches Publisher Pubmed

Aghaeebakhtiari SH^{1, 4, 10} ; Arefian E^{2, 4} ; Naderi M^{3, 4} ; Noorbakhsh F⁵ ; Nodouzi V¹ ; Asgari M^{6, 7} ; Fardesfahani P⁸ ; Mahdian R¹ ; Soleimani M⁹

Show Affiliations

1. Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, St, Tehran, Iran
2. Department of Microbiology, School of Biology, College of Science, University of Tehran, Enqelab St, Tehran, Iran
3. Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Jalal Ale Ahmad Highway, Tehran, Iran
4. Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, No. 9 East 2nd St, Saadat Abad St, Tehran, Iran
5. Department of Immunology, Tehran University of Medical Sciences, Enqelab St, Tehran, Iran
6. Oncopathology Research Center, Iran University of Medical Science (IUMS), Hemmat Highway, Tehran, Iran
7. Pathology Department of Hasheminejad Kidney Center, Iran University of Medical Sciences, Valiasr St, Tehran, Iran
8. Department of Biochemistry, Pasteur Institute of Iran, No. 358, 12th Farwardin Ave, Jomhhoori St, Tehran, Iran
9. Department of Hematology, Tarbiat Modares University, Jalal Ale Ahmad Highway, Tehran, Iran
10. Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Vakilabad Highway, Mashhad, Iran

Source: Tumor Biology Published:2015

Abstract

The long-lasting inadequacy of existing treatments for prostate cancer has led to increasing efforts for developing novel therapies for this disease. MicroRNAs (miRNAs) are believed to have considerable therapeutic potential due to their role in regulating gene expression and cellular pathways. Identifying miRNAs that efficiently target genes and pathways is a key step in using these molecules for therapeutic purposes. Moreover, computational methods have been devised to help identify candidate miRNAs for each gene/pathway. MAPK and JAK/STAT pathways are known to have essential roles in cell proliferation and neoplastic transformation in different cancers including prostate cancer. Herein, we tried to identify miRNAs that target these pathways in the context of prostate cancer as therapeutic molecules. Genes involved in these pathways were analyzed with various algorithms to identify potentially targeting miRNAs. miR-23a and miR-23b were then selected as the best potential candidates that target a higher number of genes in these pathways with greater predictive scores. We then analyzed the expression of candidate miRNAs in LNCAP and PC3 cell lines as well as prostate cancer clinical samples. miR-23a and miR-23b showed a significant downregulation in cell line and tissue samples, a finding which is consistent with overactivation of these pathways in prostate cancer. In addition, we overexpressed miR-23a and miR-23b in LNCAP and PC3 cell lines, and these two miRNAs decreased IL-6R expression which has a critical role in these pathways. These results suggest the probability of utilizing miR-23a and miR-23b as therapeutic targets for the treatment of prostate cancer. © 2015, International Society of Oncology and BioMarkers (ISOBM).

Related Docs

1. Micrornas As Biomarkers for Early Diagnosis, Targeting and Prognosis of Prostate Cancer, Pathology Research and Practice (2023)

2. Potential Role of Mir-214 in Β-Catenin Gene Expression Within Hepatocellular Carcinoma, Molecular Biology Reports (2020)

3. Poly(A) Tale: From a to A; Rna Polyadenylation in Prokaryotes and Eukaryotes, Wiley Interdisciplinary Reviews: RNA (2024)

4. Identifying Functional Cancer-Specific Mirna-Mrna Interactions in Testicular Germ Cell Tumor, Journal of Theoretical Biology (2016)

5. Combining Supervised and Unsupervised Learning for Improved Mirna Target Prediction, IEEE/ACM Transactions on Computational Biology and Bioinformatics (2018)

6. Reproducible and Reliable Real-Time Pcr Assay to Measure Mature Form of Mir-141, Applied Immunohistochemistry and Molecular Morphology (2016)

7. Multi-Sensitive Functionalized Niosomal Nanocarriers for Controllable Gene Delivery in Vitro and in Vivo, Cancer Nanotechnology (2023)

8. Exosomal Mirnas: Novel Players in Viral Infection, Epigenomics (2020)

Experts (# of related papers)

View all Related Experts

Mohammad Hossein Modarressi (2)

Hamid Reza Mirzaei (1)

Style	Citing Format
MLA	Aghaeebakhtiari SH, et al.. "Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches." Tumor Biology, vol. 36, no. 6, 2015, pp. 4203-4212.
APA	Aghaeebakhtiari SH, Arefian E, Naderi M, Noorbakhsh F, Nodouzi V, Asgari M, Fardesfahani P, Mahdian R, Soleimani M (2015). Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches. Tumor Biology, 36(6), 4203-4212.
Chicago	Aghaeebakhtiari SH, Arefian E, Naderi M, Noorbakhsh F, Nodouzi V, Asgari M, Fardesfahani P, Mahdian R, Soleimani M. "Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches." Tumor Biology 36, no. 6 (2015): 4203-4212.
Harvard	Aghaeebakhtiari SH et al. (2015) 'Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches', Tumor Biology, 36(6), pp. 4203-4212.
Vancouver	Aghaeebakhtiari SH, Arefian E, Naderi M, Noorbakhsh F, Nodouzi V, Asgari M, et al.. Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches. Tumor Biology. 2015;36(6):4203-4212.
BibTex	@article{ author = {Aghaeebakhtiari SH and Arefian E and Naderi M and Noorbakhsh F and Nodouzi V and Asgari M and Fardesfahani P and Mahdian R and Soleimani M}, title = {Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches}, journal = {Tumor Biology}, volume = {36}, number = {6}, pages = {4203-4212}, year = {2015} }
RIS	TY - JOUR AU - Aghaeebakhtiari SH AU - Arefian E AU - Naderi M AU - Noorbakhsh F AU - Nodouzi V AU - Asgari M AU - Fardesfahani P AU - Mahdian R AU - Soleimani M TI - Mapk and Jak/Stat Pathways Targeted by Mir-23A and Mir-23B in Prostate Cancer: Computational and in Vitro Approaches JO - Tumor Biology VL - 36 IS - 6 SP - 4203 EP - 4212 PY - 2015 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract