Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold

Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold Publisher Pubmed

Izanlou S¹ ; Afshar A² ; Zare A³ ; Zhilisbayeva KR⁴ ; Bakhshalizadeh S^{5, 6} ; Safaei Z⁷ ; Sehatbakhsh S⁸ ; Khaledi S¹ ; Asgari HR¹ ; Kazemnejad S⁹ ; Ajami M¹⁰ ; Ajami M¹⁰ ; Dehghan Tarzjani M¹² ; Najafzadeh V¹³ Show All Authors

Izanlou S¹
Afshar A²
Zare A³
Zhilisbayeva KR⁴
Bakhshalizadeh S^{5, 6}
Safaei Z⁷
Sehatbakhsh S⁸
Khaledi S¹
Asgari HR¹
Kazemnejad S⁹
Ajami M¹⁰
Ajami M¹⁰
Dehghan Tarzjani M¹²
Najafzadeh V¹³
Kouchakian MR¹
Mussin NM¹⁴
Kaliyev AA¹⁴
Aringazina RA¹⁵
Mahdipour M^{16, 17}
Shirazi R^{1, 18}
Tamadon A^{3, 4}

Show Affiliations

1. Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
3. PerciaVista R&D Co., Shiraz, Iran
4. Department of Scientific Work, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
5. Reproductive Development, Murdoch Children's Research Institute, Melbourne, VIC, Australia
6. Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia
7. Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, 97239, OR, United States
8. Department of Anatomical Sciences, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
9. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
10. Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran
11. Department of Hematology, Faculty of Paramedical Sciences, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
12. Department of Gynecology and Obstetrics, Imam Khomeinin Hospital, Tehran University of Medical Sciences, Tehran, Iran
13. Queensland Fertility Group (QFG), Mackay, QLD, Australia
14. General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
15. Department of Internal Medicine No. 1, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
16. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
17. Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
18. Department of Anatomy, School of Biomedical Sciences, Medicine & Health, UNSW Sydney, Sydney, Australia

Source: Tissue and Cell Published:2023

Abstract

Three-dimensional nanofiber scaffolds offer a promising method for simulating in vivo conditions within the laboratory. This study aims to investigate the influence of a bilayer amniochorionic membrane/nanofibrous fibroin scaffold on the differentiation of human menstrual blood mesenchymal stromal/stem cells (MenSCs) into female germ cells. MenSCs were isolated and assigned to four culture groups: (i) MenSCs co-cultured with granulosa cells (GCs) using the scaffold (3D-T group), (ii) MenSCs using the scaffold alone (3D-C group), (iii) MenSCs co-cultured only with GCs (2D-T group), and (iv) MenSCs without co-culture or scaffold (2D-C group). Both MenSCs and GCs were independently cultured for two weeks before co-culturing was initiated. Flow cytometry was employed to characterize MenSCs based on positive markers (CD73, CD90, and CD105) and negative markers (CD45 and CD133). Additionally, flow cytometry and immunocytochemistry were used to characterize the GCs. Differentiated MenSCs were analyzed using real-time PCR and immunostaining. The real-time PCR results demonstrated significantly higher levels of VASA expression in the 3D-T group compared to the 3D-C, 2D-T, and 2D-C groups. Similarly, the SCP3 mRNA level in the 3D-T group was notably elevated compared to the 3D-C, 2D-T, and 2D-C groups. Moreover, the expression of GDF9 was significantly higher in the 3D-T group when compared to the 3D-C, 2D-T, and 2D-C groups. Immunostaining results revealed a lack of signal for VASA, SCP3, or GDF9 markers in the 2D-T group, while some cells in the 3D-T group exhibited positive staining for all these proteins. These findings suggest that the combination of a bilayer amniochorionic membrane/nanofibrous fibroin scaffold with co-culturing GCs facilitates the differentiation of MenSCs into female germ cells. © 2023 Elsevier Ltd

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Style	Citing Format
MLA	Izanlou S, et al.. "Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold." Tissue and Cell, vol. 85, no. , 2023, pp. -.
APA	Izanlou S, Afshar A, Zare A, Zhilisbayeva KR, Bakhshalizadeh S, Safaei Z, Sehatbakhsh S, Khaledi S, Asgari HR, Kazemnejad S, Ajami M, Ajami M, Dehghan Tarzjani M, Najafzadeh V, Kouchakian MR, Mussin NM, Kaliyev AA, Aringazina RA, Mahdipour M, ... Tamadon A (2023). Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold. Tissue and Cell, 85(), -.
Chicago	Izanlou S, Afshar A, Zare A, Zhilisbayeva KR, Bakhshalizadeh S, Safaei Z, Sehatbakhsh S, et al.. "Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold." Tissue and Cell 85, no. (2023): -.
Harvard	Izanlou S et al. (2023) 'Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold', Tissue and Cell, 85(), pp. -.
Vancouver	Izanlou S, Afshar A, Zare A, Zhilisbayeva KR, Bakhshalizadeh S, Safaei Z, et al.. Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold. Tissue and Cell. 2023;85():-.
BibTex	@article{ author = {Izanlou S and Afshar A and Zare A and Zhilisbayeva KR and Bakhshalizadeh S and Safaei Z and Sehatbakhsh S and Khaledi S and Asgari HR and Kazemnejad S and Ajami M and Ajami M and Dehghan Tarzjani M and Najafzadeh V and Kouchakian MR and Mussin NM and Kaliyev AA and Aringazina RA and Mahdipour M and Shirazi R and Tamadon A}, title = {Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold}, journal = {Tissue and Cell}, volume = {85}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Izanlou S AU - Afshar A AU - Zare A AU - Zhilisbayeva KR AU - Bakhshalizadeh S AU - Safaei Z AU - Sehatbakhsh S AU - Khaledi S AU - Asgari HR AU - Kazemnejad S AU - Ajami M AU - Ajami M AU - Dehghan Tarzjani M AU - Najafzadeh V AU - Kouchakian MR AU - Mussin NM AU - Kaliyev AA AU - Aringazina RA AU - Mahdipour M AU - Shirazi R AU - Tamadon A TI - Enhancing Differentiation of Menstrual Blood-Derived Stem Cells Into Female Germ Cells Using a Bilayer Amniotic Membrane and Nano-Fibrous Fibroin Scaffold JO - Tissue and Cell VL - 85 IS - SP - EP - PY - 2023 ER -

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