Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Role of Transferrin Receptor in the Helicobacter Pylori Pathogenesis; L-Ferritin As a Novel Marker for Intestinal Metaplasia Publisher Pubmed



Hamedi Asl D1 ; Naserpour Farivar T2 ; Rahmani B1 ; Hajmanoochehri F3 ; Emami Razavi AN4 ; Jahanbin B5 ; Soleimani Dodaran M6 ; Peymani A2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Molecular Medicine, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
  2. 2. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
  3. 3. Department of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran
  4. 4. Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Pathology, Cancer Research Institute, Tehran University of Medical, Tehran, Iran
  6. 6. Department of Pathology and Molecular Medicine, Mehr Lab, Hashtgerd, Iran

Source: Microbial Pathogenesis Published:2019


Abstract

Helicobacter pylori growth requirements is a prerequisite to invade gastric epithelium and the process of injury to gastric cells will eventually lead to gastric cancer. The aim of this study is to investigate the effect of iron challenge on the expression of genes involved in iron homeostasis. The presence of Phosphoglucosamine mutase (glmM), cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) genes and mRNA expression of Iron Regulatory Protein 2 (IRP2), Transferrin Receptor (TFRC) and Ferritin Light Chain (FTL) genes in samples of 28 normal gastric mucosa, 33 chronic gastritis, 29 gastritis with intestinal metaplasia, 29 intestinal type adenocarcinoma patients were examined by real-time PCR. Immunohistochemistry was used to analyze cellular localization and protein levels. In the all H. pylori positive tissues, particularly in the basal regions of foveolar cells, TFRC was overexpressed (P < 0.05), and regardless of the H. pylori infection, FTL was overexpressed in all patient, exclusively in metaplastic glandular cells (P < 0.05). Furthermore, overexpression of IRP2 was associated with H. pylori positive chronic gastritis and intestinal metaplasia (P < 0.05). Our findings confirm the role of transferrin receptor in H. pylori attachment into the gastric mucosa to capture iron. Overexpression of FTL gene in metaplastic cells could be considered as a research background to investigate the role of this gene in the differentiation of gastric cells into intestinal metaplasia. In addition, this gene could be suggested as a diagnostic marker to be included among the other markers routinely performed by clinical diagnostic laboratories. © 2018 Elsevier Ltd
Related Docs
1. Transferrin Receptor-Mediated Liposomal Drug Delivery: Recent Trends in Targeted Therapy of Cancer, Expert Opinion on Drug Delivery (2022)
2. Occurrence of Helicobacter Pylori and Its Major Virulence Genotypes in Dental Plaque Samples of Patients With Chronic Periodontitis in Iran, Gastroenterology and Hepatology from Bed to Bench (2017)
3. The Interplay Between Aryl Hydrocarbon Receptor, H. Pylori, Tryptophan, and Arginine in the Pathogenesis of Gastric Cancer, International Reviews of Immunology (2022)
4. Association Between Gastric Cancer and the Intake of Different Types of Iron and Meats, BMC Nutrition (2023)
5. Clinical Relevance of Helicobacter Pylori Virulence Factors in Iranian Patients With Gastrointestinal Diseases, Microbial Pathogenesis (2016)
6. Inverse Association Between Dietary Iron Intake and Gastric Cancer: A Pooled Analysis of Case‐Control Studies of the Stop Consortium, Nutrients (2022)
Experts (# of related papers)
View all Related Experts
Reza Malekzadeh (1)
Nima Rezaei (1)